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Nitrogenase activity in composting horse bedding and leaves

机译：堆肥马匹和叶子中的氮酶活性

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To date little or no nitrogen fixation has been found in composting materials. However, some materials have high C:N ratios and thus might be expected to support nitrogen fixation. We examined windrows of composting horse bedding and leaves for nitrogenase activity via acetylene reduction and used carbon monoxide controls to differentiate ethylene evolved by nitrogenase from that evolved by endogenous processes. In both piles temperatures were substantially elevated and partial pressures of oxygen greatly reduced thus indicating vigorous microbial activity. In the horse bedding, there were only low rates of ethylene evolution and little of this was due to nitrogen fixation. In contrast, much higher rates of ethylene evolution were measured in the leaf pile and 94% of this was due to nitrogenase activity. We estimate that the leaf pile fixed approximately 0.062 mg N/g leaves during a 5-week period.
机译：迄今为止，在堆肥材料中几乎没有或没有发现固氮作用。但是，某些材料的C：N比很高，因此有望支持固氮。我们检查了堆肥马匹堆肥和叶堆中通过乙炔还原而产生的固氮酶活性，并使用了一氧化碳控制来区分由固氮酶演化出的乙烯与内源性过程演化出的乙烯。在这两个堆中，温度都显着升高，氧气的分压大大降低，从而表明微生物活性旺盛。在马床上，只有很少的乙烯释放速率，而其中很少是由于固氮作用。相反，在叶堆中测得的乙烯释放速率要高得多，其中94％是由于固氮酶的活性。我们估计叶堆在5周内固定了约0.062 mg N / g的叶子。

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