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首页> 外文期刊>Plant and Soil >Identification and expression studies of a catalase and a bifunctional catalase-peroxidase in Frankia strain R43
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Identification and expression studies of a catalase and a bifunctional catalase-peroxidase in Frankia strain R43

机译：Frankia菌株R43中过氧化氢酶和双功能过氧化氢酶过氧化物酶的鉴定和表达研究

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摘要

A monofunctional catalase and a bifunctional catalase-peroxidase were revealed by activity staining of nondenaturing PAGE in Frankia strain R43. Both enzymes were shown to be cytoplasmatic, growth regulated and expressed mainly during the stationary growth phase. Nevertheless, low levels of constitutive expression could also be detected during the early stages of growth. Immunoblot analyses using a polyclonal antibody raised against a catalase-peroxidase purified from Streptomyces reticuli showed a band of 83.2 kDa, with the same growth dependent pattern as obtained by the non-denaturing PAGE analyses. Induction studies revealed that both enzymes were strongly induced by raising the intracellular concentration of H2O2 with paraquat, but not with exogenous H2O2. In addition, no acquisition of tolerance to exogenous H2O2 was observed whatever the pretreatment of the inocula, i.e. despite the expression level of both hydroperoxidases.
机译：通过弗兰肯菌属菌株R43中非变性PAGE的活性染色揭示了单功能过氧化氢酶和双功能过氧化氢酶-过氧化物酶。两种酶均显示为细胞质，主要在静止生长期受生长调节和表达。然而，在生长的早期也可以检测到低水平的组成型表达。使用针对网状链霉菌纯化的过氧化氢酶过氧化物酶的多克隆抗体进行的免疫印迹分析显示，条带为83.2 kDa，具有与通过非变性PAGE分析获得的相同的生长依赖性模式。诱导研究表明，百草枯可提高细胞内过氧化氢的浓度，从而强烈诱导两种酶，而外源性过氧化氢则不会。另外，无论接种物的预处理如何，即尽管两种氢过氧化物酶的表达水平，都未观察到对外源H 2 O 2的耐受性。

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