• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant and Soil >Molecular cloning and characterization of adr and ivd genes from Frankia EuIK1 strain
【24h】

Molecular cloning and characterization of adr and ivd genes from Frankia EuIK1 strain

机译：Frankia EuIK1菌株adr和ivd基因的分子克隆和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nucleotide sequences of adrenodoxin reductase (adr) gene and isovaleryl-CoA dehydrogenase (ivd) gene and the expression pattern of adr from Frankia EuIK1 strain, symbiont of Elaeagnus umbellata, were determined. 5.5-kb NotI, 5.5-kb SacI, 1.3-kb SacI restriction fragments of pEuAR1, a cosmid clone hybridized with a squalenehopene cyclase (shc) DNA probe, were subcloned and partially sequenced. Sequence analysis showed three fragments to overlap and harbor adr and ivd genes but not the targeted shc gene. The deduced amino acid sequence of AdR, consisting of 487 amino acids, showed sequence similarity of about 55% with other AdRs, and that of ivd, consisting of 384 amino acids, showed about 60% similarity with others. RT-PCR experiments revealed that the expression of adr was in low level at 6 weeks after inoculation (WAI), reached peak at 8 WAI, and decreased to some extent at 10 WAI. AdR is a probable redox partner of [2Fe- 2S] ferredoxin involved in the biosynthesis of Fe/S cluster for cellular Fe/S proteins and also could be involved in nitrogen-fixation. It is the first report about adr and ivd in Frankia.
机译：确定肾上腺毒素还原酶（adr）基因和异戊酰辅酶A脱氢酶（ivd）基因的核苷酸序列，以及伞形螨（Elaeagnus umbellata）的共生体Frankia EuIK1菌株的adr表达模式。亚克隆pEuAR1的5.5-kb NotI，5.5-kb SacI，1.3-kb SacI限制性片段，该片段是与角鲨烯戊环化酶（shc）DNA探针杂交的粘粒克隆。序列分析显示三个片段重叠并带有adr和ivd基因，但没有靶向的shc基因。推导的由487个氨基酸组成的AdR氨基酸序列显示与其他AdR的序列相似性约为55％，而由384个氨基酸组成的ivd的氨基酸序列显示与其他AdR的相似性约为60％。 RT-PCR实验表明，adr的表达在接种后6周（WAI）处于低水平，在8 WAI时达到峰值，在10 WAI时有所下降。 AdR是[2Fe-2S]铁氧还蛋白的可能氧化还原伙伴，参与细胞Fe / S蛋白的Fe / S簇的生物合成，也可能参与固氮。这是Frankia中有关adr和ivd的第一份报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号