...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant and Soil >Host-specific plant signal and G-protein activator, mastoparan, trigger differentiation of zoospores of the phytopathogenic oomycete Aphanomyces cochlioides
【24h】

Host-specific plant signal and G-protein activator, mastoparan, trigger differentiation of zoospores of the phytopathogenic oomycete Aphanomyces cochlioides

机译：宿主特有的植物信号和G蛋白激活剂，马索帕兰，触发植物致病卵菌卵圆孔菌的游动孢子分化

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We found that the gradient of a host-specific attractant, cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone) isolated from the roots of spinach triggered encystment followed by germination of zoospores of Aphanomyces cochlioides at a concentration less than micromolar order. This compound did not affect the growth and reproduction of this phytopathogen up to 10(-6) M concentration in the culture medium. We also observed that mastoparan, an activator of heterotrimeric G-protein could inhibit the motility of zoospores and then strikingly effect encystment followed by 60 - 80% germination of cysts. Concomitant application of cochliophilin A and mastoparan showed stronger encystment followed by 100% germination of cysts. In addition, we have observed that chemicals interfering with phospholipase C activity ( neomycin) and Ca2+ influx/release ( EGTA and loperamide) suppress cochliophilin A or mastoparan induced encystment and germination. These results suggest that G-protein mediated signal transduction mechanism may be involved in the differentiation of the A. cochlioides zoospores. This is the first report on the differentiation of oomycete zoospores initiated by a host-specific plant signal or a G-protein activator.
机译：我们发现，从菠菜根部分离的宿主特异性引诱剂球菌亲和素A（5-羟基-6,7-亚甲基二氧基黄酮）的梯度触发了包囊，随后以低于微摩尔级的浓度萌发了香豆菌的游动孢子。该化合物在培养基中浓度不超过10（-6）M时不会影响该植物病原体的生长和繁殖。我们还观察到，异三聚体G蛋白的激活剂马索巴兰可以抑制游动孢子的运动，然后显着地影响包囊，随后囊肿萌发60-80％。伴有球蛋白A和乳脂蛋白的应用显示出更强的包囊性，随后囊肿100％发芽。此外，我们已经观察到，干扰磷脂酶C活性（新霉素）和Ca2 +流入/释放（EGTA和洛哌丁胺）的化学物质会抑制球蛋白A或乳脂素诱导的包囊和萌发。这些结果表明G蛋白介导的信号转导机制可能参与了A.cochlioides游动孢子的分化。这是关于由宿主特异性植物信号或G蛋白激活剂引发卵母细胞游动孢子分化的首次报道。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号