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首页> 外文期刊>Plant and Soil >Nickel tolerance and accumulation by bacteria from rhizosphere of nickel hyperaccumulators in serpentine soil ecosystem of Andaman, India
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Nickel tolerance and accumulation by bacteria from rhizosphere of nickel hyperaccumulators in serpentine soil ecosystem of Andaman, India

机译：印度安达曼蛇形土壤生态系统中镍超累积物对根际细菌的镍耐受性和累积

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摘要

Rhizosphere microorganisms harboring nickel hyperaccumulators, iRinorea bengalensis (Wall.) O. K. and iDichapetalum gelonioides ssp. iandamanicum (King) Leenh. endemic to serpentine outcrops of Andaman Islands, India, were screened for their tolerance and accumulation of Ni. The rhizosphere soils from both the plants were rich in total and available Ni along with Co, Cr, Fe and Mg but poor in microbial density and were dominated by bacteria. Out of total 123 rhizosphere microorganisms (99 bacteria and 24 fungi), bacteria were more tolerant to Ni than fungi. Viable cells of selected Ni-tolerant bacterial isolates (MIC = 13.6-28.9 mM Ni) belonging to iPseudomonas, iBacillus and iCupriavidus were capable of accumulating nickel (209.5-224.0 ^kM Ni gu-1 protein) from aqueous solution. iCupriavidus pauculus KPS 201 (MTCC 6280), showing highest degree of nickel tolerance (MIC 28.9 mM Ni) and uptake (224.0 ^kM Ni gu-1 protein, 60 min) was used for detailed study. Kinetics of nickel uptake in iC. pauculus KPS 201 followed a linearized Lineweaver-Burk plot. The iKdm and iVdmax for nickel uptake by minimal medium grown-cells approximated 1.5 mM Ni and 636.9 ^kM Ni gu-1 protein, respectively. The uptake process was inhibited by Co, Cu, Cd, Mg, Mn and Zn, however, complete inhibition was not achieved even in presence of 500 mM Mg. Metabolic inhibitors, sodium azide (1.0 mM) and carbonyl cyanide im-chlorophenylhydrazone (0.4 mM) strongly inhibited nickel uptake suggesting the process as an energy dependent one. The present study clearly shows that bacteria in the rhizosphere of Ni-hyperaccumulators are capable of tolerating high concentration of Ni and also possesses nickel uptake potential. The Ni-hyperaccumulators in combination with these Ni-resistant bacteria could be an ideal tool for nickel bioremediation.
机译：带有镍超蓄积物，孟加拉iRinorea Bengalalensis（Wall.O.K.）和iDichapetalum gelonioides ssp的根际微生物。 iandamanicum（国王）Leenh。对印度安达曼群岛的蛇形露头特有种进行了筛查，以了解它们对镍的耐受性和积累。两种植物的根际土壤都含有丰富的总氮和可利用的镍以及钴，铬，铁和镁，但微生物密度较弱，且以细菌为主。在123种根际微生物（99种细菌和24种真菌）中，细菌对Ni的耐受性要强于真菌。选自iPseudomonas，iBacillus和iCupriavidus的选定的耐Ni细菌分离株（MIC = 13.6-28.9 mM Ni）的活细胞能够从水溶液中累积镍（209.5-224.0 kM Ni gu-1蛋白）。 iCupriavidus pauculus KPS 201（MTCC 6280）显示出最高的耐镍性（MIC 28.9 mM Ni）和吸收（224.0 ^ kM Ni gu-1蛋白，60分钟）用于详细研究。 iC中镍吸收的动力学。丘疹KPS 201遵循线性化的Lineweaver-Burk图。最小培养基生长细胞摄取镍的iKdm和iVdmax分别约为1.5 mM Ni和636.9 ^ kM Ni gu-1蛋白。吸收过程受到Co，Cu，Cd，Mg，Mn和Zn的抑制，但是，即使存在500 mM Mg，也无法完全抑制。代谢抑制剂叠氮化钠（1.0 mM）和羰基氰酰亚胺-氯苯基hydr（0.4 mM）强烈抑制了镍的吸收，表明该过程是一种依赖能量的过程。本研究清楚地表明，Ni-超级蓄积器的根际细菌能够耐受高浓度的Ni，并且还具有镍吸收的潜力。与这些耐镍细菌结合的镍超蓄积剂可能是进行镍生物修复的理想工具。

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