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首页> 外文期刊>Plant and Soil >Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates
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Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates

机译：Pantoea agglomerans根际分离株的生产吲哚-3-乙酸，芳香族氨基酸氨基转移酶活性和促进植物生长

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The production of auxins, such as indole-3-acetic acid (IAA), by rhizobacteria has been associated with plant growth promotion, especially root initiation and elongation. Six indole-producing bacteria isolated from the rhizosphere of legumes grown in Saskatchewan soils and identified as Pantoea agglomerans spp. were examined for their ability to promote the growth of canola, lentil and pea under gnotobiotic conditions and for tryptophan (Trp)-dependent IAA production. Five of the isolates enhanced root length, root weight or shoot weight by 15-37% in at least one of the plant species, but isolates 3-117 and 5-51 were most consistent in enhancing plant growth across the three species. Indole concentrations in the rhizosphere of plants grown under gnotobiotic conditions increased in the presence of the rhizosphere isolates and when Trp was added 3 days prior to plant harvest. Isolates 3-117, 5-51 and 5-105 were most effective in increasing rhizosphere indole concentrations. Colony hybridization confirmed that all of the isolates possessed the ipdC gene which codes for a key enzyme in the Trp-dependent IAA synthetic pathway. The activity of amino acid aminotransferase (AAT), catalyzing the first step in the Trp-dependent synthetic pathway, was examined in the presence of Trp and other aromatic amino acids. All of the isolates accumulated Trp internally and released different amounts of IAA. The production of IAA from the isolates was greatest in the presence of Trp, ranging from 2.78 to 16.34 mu g mg protein super(-1) in the presence of 250 mu g of Trp ml super(-1). The specific activity of AAT was correlated with the concentration of IAA produced in the presence of Trp but not when tyrosine (Tyr), phenylalanine (Phe) or aspartate (Asp) was used as a sole nitrogen source. Isolate 3-117, which produced significant concentrations of IAA in the presence and absence of Trp, was able to use aromatic amino acids as sole sources of nitrogen and was most consistent in enhancing the growth of canola, lentil and pea may have potential for development as a plant growth-promoting inoculant.
机译：根瘤菌产生的生长素，例如吲哚-3-乙酸（IAA），与植物的生长促进有关，特别是根的萌生和伸长。从萨斯喀彻温省土壤中生长的豆类根际分离出了六种产生吲哚的细菌，并被鉴定为Pantoea agglomerans spp。检查了它们在促生条件下促进油菜籽，小扁豆和豌豆生长的能力，以及依赖色氨酸（Trp）的IAA产生的能力。在至少一种植物物种中，有五个分离株的根长，根重或苗重增加了15-37％，但是在三个物种中，分离株3-117和5-51在提高植物生长方面最一致。在有根际分离株的存在下，以及在植物收获前三天加入Trp时，在生生物条件下生长的植物根际中的吲哚浓度会增加。分离株3-117、5-51和5-105对增加根际吲哚浓度最有效。菌落杂交证实所有分离株均具有ipdC基因，该基因编码Trp依赖性IAA合成途径中的关键酶。在存在Trp和其他芳香族氨基酸的情况下，检查了催化Trp依赖性合成途径第一步的氨基酸氨基转移酶（AAT）的活性。所有分离株在内部积累了Trp，并释放了不同量的IAA。在存在Trp的情况下，分离株的IAA产量最高，在存在250μgTrp ml super（-1）的情况下，蛋白质的Super（-1）范围为2.78至16.34μg。 AAT的比活性与存在Trp时产生的IAA浓度相关，但与酪氨酸（Tyr），苯丙氨酸（Phe）或天冬氨酸（Asp）用作唯一氮源无关。分离物3-117在存在和不存在Trp的情况下均产生大量IAA，能够将芳香族氨基酸用作唯一的氮源，并且最能促进油菜籽，扁豆和豌豆的生长作为促进植物生长的孕育剂。

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