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Water-extractable humic substances alter root development and epidermal cell pattern in Arabidopsis

机译：可水提取的腐殖质改变拟南芥的根系发育和表皮细胞模式

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摘要

The effect of a low-molecular weight, water-extractable fraction of humic substances (WEHS) derived from sphagnum peat on post-embryonic plant development has been studied using Arabidopsis roots. Application of humic substances caused an array of changes in root morphology, such as an increase in root hair length and density, formation of ectopic root hairs, and an increase in cell proliferation in the root ground tissue. Application of WEHS affected genes involved in epidermal cell fate specification, suggesting that humic substances can alter developmental programs at an early stage of root cell differentiation. The WEREWOLF and GLABRA2 genes, encoding negative regulators of the root hair cell fate, were significantly down-regulated in the presence of WEHS. Thus, the presence of humic substances caused an ordered remodeling of the root morphology, leading to an increased absorptive surface of the root. Growth in the presence of WEHS did not rescue the phenotype of the root hair defective rhd6 mutant. Analyzing BA3:uidA and DR5:uidA transgenic plants, carrying auxin response elements, and monitoring the expression of the auxin-responsive GH3 gene by real-time RT-PCR did not provide evidence for a WEHS-induced expression of auxin-related genes. It is concluded that WEHS do not exert their effects in an auxin-like manner.
机译：已经使用拟南芥根研究了低分子量，可水提取的泥炭藓泥炭腐殖质（WEHS）对胚后植物发育的影响。施用腐殖质会导致根部形态发生一系列变化，例如根毛长度和密度的增加，异位根毛的形成以及根基组织中细胞增殖的增加。 WEHS的应用影响了表皮细胞命运规范中涉及的基因，这表明腐殖质可以在根细胞分化的早期改变发育程序。在存在WEHS的情况下，编码根毛细胞命运的负调控因子的WEREWOLF和GLABRA2基因被显着下调。因此，腐殖质的存在导致根部形态的有序重塑，导致根部吸收表面的增加。 WEHS存在下的生长不能挽救根毛缺陷型rhd6突变体的表型。分析带有生长素应答元件的BA3：uidA和DR5：uidA转基因植物，并通过实时RT-PCR监测生长素应答性GH3基因的表达，并没有提供WEHS诱导的生长素相关基因表达的证据。结论是，WEHS不会以生长素样的方式发挥作用。

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