• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Disease >Development of a Greenhouse Screening Method for Adult Plant Response in Wheat to Stem Rust
【24h】

Development of a Greenhouse Screening Method for Adult Plant Response in Wheat to Stem Rust

机译：小麦成年植物对茎锈病反应的温室筛选方法的建立

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Screening for adult plant resistance in wheat to stem rust, caused by Puc-cinia graminis f. sp. tririci, is generally conducted in field plots. Although such evaluations are successful if managed properly, field ratings are time consuming, expensive,weather dependent, and open to inoculum of unwanted races or other confounding diseases. The objective of this study was to develop a dependable system of screening the response of adult plants to stem rust under greenhouse conditions. A comparison of inoculation methods and incubation environments showed that plants inoculated with urediniospores suspended in water, followed by a 24 h dew period in a plastic chamber constructed in a greenhouse, gave the most consistent results. Measurements of responsetype, stem rust severity, and frequency in follow-up experiments indicated that the most reliable infection wasobtained when plants sprayed with 1.25 mg urediniospores per ml water were incubated in the plastic chamber. Using the optimized protocol, a Kariega x Avocet S doubled haploid population was inoculated with two P. graminis f. sp. tritici races. Dependingon the race, composite interval mapping showed flag leaf infection type to be significantly influenced by regions on chromosomes 6A, 6D, and 7D. Stem rust severity and reaction type mapped to chromosomes 6D and/or 6A. The Lr34/Yrl8/Sr57 gene derived from Kariega on chromosome 7D affected the rust response on flag leaves but not on stems of greenhouse-grown plants. This study showed that phenotyping and genetic analysis of especially major effect stem rust resistance in adult wheat plants is possible and reproducible under controlled conditions in a greenhouse.
机译：筛选成年小麦因锈病引起的对茎锈病的抗性f。 sp。通常在田间试验中进行。尽管如果进行适当的管理，这样的评估是成功的，但是现场评估是费时，昂贵，取决于天气的，并且易于接种有害种族或其他混杂疾病。这项研究的目的是建立一个可靠的系统，以筛选成年植物在温室条件下对茎锈病的反应。接种方法和孵化环境的比较表明，用悬浮在水中的梭菌孢子接种的植物，随后在温室中的塑料室中进行24小时的露水期，可获得最一致的结果。在后续实验中对响应类型，茎锈病严重程度和频率的测量表明，将喷洒了每毫升水1.25 mg的双孢子虫孢子的植物在塑料室内孵育后，可获得最可靠的感染。使用优化的方案，将Kariega x Avocet S加倍的单倍体种群接种了两个P. graminis f。 sp。小麦种族。根据种族，复合区间作图显示旗叶感染类型受6A，6D和7D染色体上区域的显着影响。茎锈病的严重程度和反应类型已映射到6D和/或6A号染色体。来自Kariega的7r号染色体上的Lr34 / Yrl8 / Sr57基因影响了旗叶上的锈反应，但不影响温室种植植物茎上的锈反应。这项研究表明，在温室中受控条件下，成年小麦植株尤其是茎秆抗锈性的主要作用的表型分析和遗传分析是可能的并且可重现的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号