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首页> 外文期刊>Plant Disease >Effect of Verticillium dahliae Soil Inoculum Levels on Spinach Seed Infection
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Effect of Verticillium dahliae Soil Inoculum Levels on Spinach Seed Infection

机译：黄萎病菌土壤接种量对菠菜种子感染的影响

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Verticillium dahliae is a soilborne pathogen and a threat to spinach seed production. The aim of this study was to understand the relation between V. dahliae soil inoculum and infection in harvested seed. Quantitative polymerase chain reaction was used for quantification of the pathogen. Semifield experiments in which spinach was grown in soils with different inoculum levels enabled us to determine a threshold level for V. dahliae DNA of 0.003 ng/g of soil for seed infection to occur. Soils from production fields were sampled in 2013 and 2014 during and before planting, aswell as the harvested seed. Seed from plants grown in infested soils were infected with V. dahliae in samples from both the semifield and open-field experiments. Lower levels of pathogen were found in seed from spinach grown in soils with a scattered distribution of V. dahliae (one or two positive of three soil subsamples) than in soils with a uniform distribution of the pathogen (three of three positive soil subsamples). Our results showed that infection of V. dahliae in harvested seed strongly depended on the presence of pathogen inoculum in the soil.
机译：黄萎病菌是土壤传播的病原体，对菠菜种子的生产构成威胁。这项研究的目的是了解大丽弧菌土壤接种物与收获种子感染之间的关系。定量聚合酶链反应用于病原体的定量。在不同接种水平的土壤中种植菠菜的半田间试验使我们能够确定大虫弧菌DNA的阈值水平为0.003 ng / g，以防止种子感染。在种植期间和种植之前以及2013年和2014年，对生产田的土壤以及收获的种子进行了采样。在半田和野外试验的样品中，将感染了受侵染土壤的植物的种子感染了大丽花弧菌。在散布有大隐孢子虫的土壤（三个土壤子样品中的一个或两个阳性）中生长的菠菜种子中发现的病原体水平低于均匀分布病原体的土壤（三个正土壤子样品中的三个）。我们的结果表明，收获的种子中对大丽花弧菌的感染在很大程度上取决于土壤中病原体接种物的存在。

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