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Hormone profiles in microalgae: Gibberellins and brassinosteroids

机译:微藻中的激素谱:赤霉素和油菜素甾体

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Endogenous gibberellins and brassinosteroids were quantified in 24 axenic microalgae strains from the Chlorophyceae, Trebouxiophyceae, Ulvophyceae and Charophyceae microalgae strains after 4 days in culture. This is the first report of endogenous gibberellins being successfully detected in microalgae. Between 18 and 20 gibberellins were quantified in all strains with concentrations ranging from 342.7pg mg~(-1) DW in Raphidocelis subcapitata MACC 317-4746.1pg mg~(-1) DW in Scotiellopsis terrestris MACC 44. Slower growing strains (S. terrestris MACC 44, Gyoerffyana humicola MACC 334, Nautococcus mamillatus MACC 716 and Chlorococcum ellipsoideum MACC 712) exhibited the highest gibberellin contents while lowest levels of gibberellins were found in faster growing strains (R.subcapitata MACC 317 and Coelastrum excentrica MACC 504). In all strains, the active gibberellin detected in the highest concentration was GA_6, the predominant intermediates were GA_(15) and GA_(53) and the main biosynthetic end products were GA_(13) and GA_(51). Gibberellin profiles were similar in all strains except for the presence/absence of GA_(12) and GA_(12)ald. To date this is the second report of endogenous brassinosteroids in microalgae. Brassinosteroids were detected in all 24 strains with concentrations ranging from 117.3pg mg~(-1) DW in R.subcapitata MACC 317-977.8pg mg~(-1) DW in Klebsormidium flaccidum MACC 692. Two brassinosteroids, brassinolide and castasterone were determined in all the strains. Generally, brassinolide occurred in higher concentrations than castasterone.
机译:培养4天后,在来自绿藻科,碳藻科,藻科和风铃科微藻菌株的24种轴生微藻菌株中定量内源赤霉素和油菜素类固醇。这是在微藻中成功检测到内源赤霉素的首次报道。在所有菌株中定量了18至20种赤霉素,其浓度在小黑麦蚜MACC中的浓度为342.7pg mg〜(-1)DW,在陆生拟南芥MACC 44中为317-4746.1pg mg〜(-1)DW。生长缓慢的菌株(S.陆地藜麦MACC 44,Gyoerffyana humicola MACC 334,Nautococcus mamillatus MACC 716和Elidesoideum ellipsoideum MACC 712的赤霉素含量最高,而生长最快的菌株(R.subcapitata MACC 317和Coelastrum excentrica)的赤霉素含量最低。在所有菌株中,最高浓度的活性赤霉素为GA_6,主要中间体为GA_(15)和GA_(53),主要生物合成终产物为GA_(13)和GA_(51)。除了是否存在GA_(12)和GA_(12)ald外,所有菌株中的赤霉素谱均相似。迄今为止,这是微藻中内源性油菜素类固醇的第二次报道。在24株菌株中检测到油菜素类固醇,其浓度在短杆菌KAC 317-977.8pg mg〜(-1)DW中的含量为117.3pg mg〜(-1)DW。测定了两种油菜素类固醇,油菜素内酯和蓖麻油酮。在所有的应变中。一般而言,油菜素内酯的浓度高于卡斯达松。

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