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首页> 外文期刊>Biomaterials >Crosslinked hyaluronan scaffolds as a biologically active carrier for valvular interstitial cells.
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Crosslinked hyaluronan scaffolds as a biologically active carrier for valvular interstitial cells.

机译：交联的透明质酸支架作为瓣膜间质细胞的生物活性载体。

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摘要

Hyaluronic acid (HA), a major component of the cardiac jelly during heart morphogenesis, is a polysaccharide that upon modification can be photopolymerized into hydrogels. Previous work in our lab has found that photopolymerizable HA hydrogels are suitable scaffolds for the culture and proliferation of valvular interstitial cells (VICs), the most prevalent cell type in native heart valves. The physical properties of HA gels are easily modified through alteration in material crosslink density or by copolymerizing with other reactive macromolecules. Degradation products of HA gels and the starting macromers significantly increased VIC proliferation when added to cell cultures. With low molecular weight HA (<6700 Da) exhibiting greatest stimulation of VIC proliferation. Low molecular weight HA degradation products added to VIC cultures also resulted in a four-fold increase in total matrix production and a two-fold increase in elastin production over untreated controls. VIC internalization of HA, as shown by cellular uptake of fluorescently labeled HA, likely activates signaling cascades resulting in the biological responses seen here. Lastly, VICs encapsulated within HA hydrogels remained viable, and significant elastin production was observed after 6 weeks of culture. This work shows promise for the creation of a tissue-engineered heart valve utilizing the synergistic relationship between hyaluronic acid and VICs.
机译：透明质酸（HA）是心脏形态发生过程中the胶的主要成分，是一种多糖，经修饰后可以光聚合成水凝胶。我们实验室以前的工作发现，可光聚合的HA水凝胶是用于瓣膜间质细胞（VIC）（天然心脏瓣膜中最普遍的细胞类型）的培养和增殖的合适支架。 HA凝胶的物理性质很容易通过改变材料的交联密度或与其他反应性大分子共聚而改变。当添加到细胞培养物中时，HA凝胶和起始大分子单体的降解产物显着增加了VIC的增殖。具有低分子量的HA（<6700 Da）表现出对VIC增殖的最大刺激。与未处理的对照相比，添加到VIC培养物中的低分子量HA降解产物还导致总基质产量增加了4倍，弹性蛋白产量增加了2倍。 HA的VIC内部化（如荧光标记的HA的细胞摄取所示）可能激活信号级联反应，从而导致此处看到的生物学反应。最后，封装在HA水凝胶中的VIC仍然可行，并且在培养6周后观察到大量的弹性蛋白产生。这项工作显示出利用透明质酸和VIC之间协同作用创建组织工程性心脏瓣膜的希望。

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