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首页> 外文期刊>Plant Pathology >First report of crown gall of apricot (Prunus armeniaca) caused by Agrobacterium tumefaciens in Turkey
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First report of crown gall of apricot (Prunus armeniaca) caused by Agrobacterium tumefaciens in Turkey

机译：土耳其根癌土壤杆菌引起的杏冠李的首次报道

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In the winter of 2002, nearly 30% of 2-year-old apricot trees (Prunus armeniaca cv Ninfa) in two commercial orchards in Adana and Mersin, in the eastern Mediterranean region of Turkey, were observed with crown gall symptoms. Tumours and galls were often found at or just below the soil surface on the roots or crown region of the apricot plants as described by Ogawa et al. (1995). A nonfluorescent, Gram-negative bacterium was isolated from diseased tissues onto King's medium B Fifteen representative strains were characterized as aerobic, nonsporing, nonpigmented, rod-shaped, oxidase-negative and catalase-positive Fatty acid analysis identified the strains as Agrobacterium tumefaciens with similarity indices ranging from 94 6 to 98.2% (Bouzar et al, 1993) Pathogenicity of the strains was tested on carrot slices in Petri dishes and 5-week-old tomato plants (cv. H-2274) by needle inoculation of bacterial suspensions containing 10~8 cfu ml~(-1) in 8 5 g L~(-1) saline. A reference strain of A tumefaciens, GSPB 7 and saline were used as positive and negative controls, respectively. Inoculated and control plants were maintained in a controlled climate room, for 20 days at 2 5 deg C and 70% RH to observe symptom development. Gall symptoms on carrot slices developed within 7-8 days and on tomato plants within 15-20 days. No symptoms developed on negative control plants. The bacterium was re-isolated from inoculated tomato plants and identified as A. tumefaciens. The occurrence of crown gall disease on rose (Rosa spp ) cultivars in Turkey has been reported previously f Aysan & Sahin, 200 3) However, this is the first report of the crown gall on apricot trees grown in Turkey. To the best of our knowledge, the apricot cuttings in both orchards were imported from outside of Turkey Therefore, it is possible that the pathogen may have been introduced into Turkey with the cuttings.
机译：在2002年冬季，在土耳其东部地中海地区的Adana和Mersin的两个商业果园中，观察到近30％的2年龄杏树（李属杏）。如Ogawa等人所述，经常在杏树的根部或冠状区域的土壤表面或土壤表面以下发现肿瘤和胆汁。 （1995）。从患病组织中分离出一种无荧光，革兰氏阴性细菌到King's B培养基上。十五个代表性菌株的特征是好氧，无孢子，无色素，杆状，氧化酶阴性和过氧化氢酶阳性。指数范围从94 6到98.2％（Bouzar et al，1993），通过针头接种含有10种细菌的悬浮液，在皮氏培养皿和5周龄的番茄植株（cv。H-2274）的胡萝卜切片上测试了菌株的致病性。在8 5 g L〜（-1）盐水中〜8 cfu ml〜（-1）。根癌农杆菌，GSPB 7和盐水的参考菌株分别用作阳性和阴性对照。将接种的植物和对照植物在可控制气候的房间中在2 5摄氏度和70％相对湿度下保持20天，以观察症状的发展。胡萝卜切片在7-8天内出现胆汁症状，番茄植物在15-20天内出现胆汁症状。在阴性对照植物上没有出现症状。从接种的番茄植物中重新分离出该细菌，并将其鉴定为根癌农杆菌。以前有报道称，土耳其玫瑰（Rosa spp）品种上发生了冠disease病（Aysan＆Sahin，200 3）。然而，这是土耳其种植的杏树上冠the病的首次报道。据我们所知，两个果园中的杏插条都是从土耳其境外进口的。因此，病原体可能随插条被引入土耳其。

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