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首页> 外文期刊>Chemical Senses >A strong nerve dependence of Sonic hedgehog expression in basal cells in mouse taste bud and an autonomous transcriptional control of genes in differentiated taste cells
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A strong nerve dependence of Sonic hedgehog expression in basal cells in mouse taste bud and an autonomous transcriptional control of genes in differentiated taste cells

机译：小鼠味蕾基底细胞中Sonic刺猬表达的强烈神经依赖性和分化味细胞中基因的自主转录控制

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The nerve-dependency of gene expression in mouse taste bud was examined through an analysis of changes in gene expression in and around the taste buds in circumvallate papillae after surgery of cranial nerve IXth (glossopharyngeal nerve). The number of cells expressing T1r3, gustducin, Mash1 and Nkx2.2 gradually decreased after denervation. However, the expression intensity of these genes was barely influenced by denervation, and strong expression was observed at 6 days after denervation. In contrast, the basal cell-specific Sonic hedgehog (Shh) expression in the taste buds was decreased markedly at 6 h after denervation. In the regeneration process of taste buds, Shh expression was observed during a very early phase before taste bud formation. These results indicate the autonomous transcriptional control of genes in differentiated taste cells and the strong nerve-dependency of Shh expression in basal cells. Furthermore, in order to reveal the mitotic activity of Shh-expressing cells in taste buds, the BrdU-labeling experiments were performed using a combination of BrdU-immunohistochemistry and in situ hybridization. BrdU-signal was very rarely observed in Shh-expressing cells immediately after BrdU injection, and the signals were noted mainly in Ptc-expressing cells. BrdU signals rapidly increased in Shh-expressing cells in following 12 h and began to decrease after 2 days post-injection. These results suggest that most Shh-expressing cells are not mitotically active, but that Shh-expressing cells may be in the early transient developmental state of taste cells in taste buds.
机译：通过分析颅神经第IXth（舌咽神经）手术后在周缘乳头中味蕾内部和周围的基因表达变化，分析了小鼠味蕾中基因表达的神经依赖性。去神经后，表达T1r3，gustgustin，Mash1和Nkx2.2的细胞数量逐渐减少。然而，这些基因的表达强度几乎不受去神经支配的影响，并且在去神经支配后6天观察到强表达。相反，去神经后6小时，味蕾中的基础细胞特异性Sonic刺猬（Shh）表达明显降低。在味蕾的再生过程中，在味蕾形成之前的很早阶段就观察到Shh表达。这些结果表明分化的味觉细胞中基因的自主转录控制和基底细胞中Shh表达的强烈神经依赖性。此外，为了揭示味蕾中表达Shh的细胞的有丝分裂活性，结合BrdU免疫组织化学和原位杂交技术进行了BrdU标记实验。 BrdU注射后立即在表达Shh的细胞中很少观察到BrdU信号，并且主要在表达Ptc的细胞中注意到该信号。在接下来的12小时内，表达Shh的细胞中BrdU信号迅速增加，并且在注射后2天后开始减少。这些结果表明大多数表达Shh的细胞不是有丝分裂活跃的，但表达Shh的细胞可能处于味蕾中味觉细胞的早期瞬时发育状态。

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