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Preparation and characterization of cationic PLGA nanospheres as DNA carriers

机译：阳离子PLGA纳米球作为DNA载体的制备与表征

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Nanoparticles formulated from biodegradable polymers such as poly(lactic acid) (PLA) and poly(lactide-co-glycolide) (PLGA) are being extensively investigated as non-viral gene delivery systems due to their controlled release characteristics and biocompatibility. PLGA nanoparticles for DNA delivery are mainly formulated by an emulsion-solvent evaporation technique using PVA as a stabilizer generating negatively charged particles and heterogeneous size distribution. The objective of the present study was to formulate cationically modified PLGA nanoparticles with defined size and shape that can efficiently bind DNA. An Emulsion-diffusion-evaporation technique to make cationic nanospheres composed of biodegradable and biocompatible co-polyester PLGA has been developed. PVA-chitosan blend was used to stabilize the PLGA nanospheres. The nanospheres were characterized by atomic force microscopy (AFM), photon-correlation spectroscopy (PCS), and Fourier transform infrared spectroscopy (FTIR). Zeta potential and gel electrophoresis studies were also performed to understand the surface properties of nanospheres and their ability to condense negatively charged DNA. The designed nanospheres have a zeta potential of 10 mV at pH 7.4 and size under 200 nm. From the gel electrophoresis studies we found that the charge on the nanospheres is sufficient to efficiently bind the negatively charged DNA electrostatically. These cationic PLGA nanospheres could serve as potential alternatives of the existing negatively charged nanoparticles. (C) 2003 Elsevier Ltd. All rights reserved. [References: 25]
机译：由可生物降解的聚合物（如聚乳酸（PLA）和聚丙交酯-乙交酯-乙交酯）（PLGA）制成的纳米颗粒由于其控释特性和生物相容性而被广泛研究为非病毒基因传递系统。用于DNA传递的PLGA纳米粒子主要通过乳液溶剂蒸发技术配制而成，使用PVA作为稳定剂，可产生带负电的粒子和不均匀的尺寸分布。本研究的目的是配制具有确定的大小和形状的阳离子修饰的PLGA纳米颗粒，可以有效地结合DNA。已经开发了一种乳液-扩散-蒸发技术来制备由可生物降解和生物相容的共聚酯PLGA组成的阳离子纳米球。 PVA-壳聚糖共混物用于稳定PLGA纳米球。通过原子力显微镜（AFM），光子相关光谱（PCS）和傅里叶变换红外光谱（FTIR）对纳米球进行了表征。还进行了Zeta电位和凝胶电泳研究，以了解纳米球的表面特性及其凝聚带负电荷的DNA的能力。设计的纳米球在pH 7.4下的Zeta电位为10 mV，尺寸在200 nm以下。通过凝胶电泳研究，我们发现纳米球上的电荷足以有效地静电结合带负电荷的DNA。这些阳离子PLGA纳米球可作为现有带负电荷纳米颗粒的潜在替代物。 （C）2003 Elsevier Ltd.保留所有权利。 [参考：25]

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