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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >TRYPANOSOMA CRUZI INFECTION IN WILD MAMMALS FROM A CHAGASIC AREA OF CHILE.
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TRYPANOSOMA CRUZI INFECTION IN WILD MAMMALS FROM A CHAGASIC AREA OF CHILE.

机译：智利乍得地区野生哺乳动物中的锥虫克鲁斯感染。

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摘要

We report results of PCR-DNA based detection of Trypanosoma cruzi in wild mammal reservoirs from a chagasic area of Chile. We analyzed 157 blood samples from wild mammals including the marsupial Thylamis elegans and the rodents Octodon degus, Phyllotis darwini, and Abrothrix olivaceus. In addition, 42 blood samples from goats (i.e., a peridomestic mammal) were analyzed. Blood samples were used to extract DNA, and PCR was performed using the amplification of minicircle DNA sequences. Southern analysis was used to confirm diagnosis with a universal probe of P(32)-labeled kinetoplast DNA. Altogether, 51% of the wild and 36% of the peridomestic mammals were infected with T. cruzi. These findings suggest that the real T. cruzi infection levels in wild and peridomiciliary reservoirs are higher than those previously determined with serological and parasitological conventional methods. The relevance of our results is discussed in term of the risk factor of infection in human.
机译：我们报告基于PCR-DNA的智利锥虫病野生哺乳动物水库中克氏锥虫的检测结果。我们分析了来自野生哺乳动物的157个血液样本，包括有袋线虫Thylamis elegans和啮齿动物Octodon degus，Phyllotis darwini和Abrothrix olivaceus。另外，分析了来自山羊（即，哺乳动物）的42份血液样品。血液样本用于提取DNA，并使用小环DNA序列的扩增进行PCR。 Southern分析被用来确认诊断的通用探针P（32）标记的运动塑料DNA。总共有51％的野生哺乳动物和36％的腹膜哺乳动物感染了曲霉。这些发现表明，野生的和周生的微生物库中的真正的克鲁氏锥虫感染水平高于以前用血清学和寄生虫学常规方法确定的水平。我们就人类感染的危险因素讨论了我们研究结果的相关性。

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