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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Fabrication of multiwalled carbon nanotubes/polyaniline modified Au electrode for ascorbic acid determination
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Fabrication of multiwalled carbon nanotubes/polyaniline modified Au electrode for ascorbic acid determination

机译：用于抗坏血酸测定的多壁碳纳米管/聚苯胺修饰金电极的制备

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摘要

An ascorbate oxidase (AsOx) (E.C.1.10.3.3) purified from Lagenaria siceraria fruit was immobilized covalently onto a carboxylated multiwalled carbon nanotubes and polyaniline (c-MWCNT/PANI) layer electrochemically deposited on the surface of an Au electrode. The diffusion coefficient of ascorbic acid was determined as 3.05 × 10~(-4) cm~2 s~(-1). The behavior of different electrolytes on electro-deposition was also studied. An ascorbate biosensor was fabricated using a AsOx/c-MWCNT/PANI/Au electrode as a working electrode, Ag/AgCl (3 M/saturated KCl) as standard and Pt wire as an auxiliary electrode connected through a potentiostat. Linear range, response time and detection limit were 2-206 μM, 2 s and 0.9 μM respectively. The biosensor showed optimum response at pH 5.8 and in a broader temperature range (30-45 °C), when polarized at +0.6 V. The biosensor was employed for determination of ascorbic acid level in sera, fruit juices and vitamin C tablets. The sensor was evaluated with 91% recovery of added ascorbic acid in sera and 6.5% and 11.4% within and between batch coefficients of variation respectively for five serum samples. There was a good correlation (r = 0.98) between fruit juice ascorbic acid values by the standard 2,6-dichlorophenolindophenol (DCPIP) method and the present method. The enzyme electrode was used 200 times over a period of two months, when stored at 4 °C. The biosensor has advantages over earlier enzyme sensors in that it has no leakage of enzyme, due to the covalent coupling of enzyme with the support, lower response time, wider working range, higher storage stability and no interference by serum substances.
机译：从紫刺槐果实中纯化的抗坏血酸氧化酶（AsOx）（E.C.1.10.3.3）被共价固定在电化学沉积在Au电极表面的羧化多壁碳纳米管和聚苯胺（c-MWCNT / PANI）层上。抗坏血酸的扩散系数确定为3.05×10〜（-4）cm〜2 s〜（-1）。还研究了不同电解质在电沉积中的行为。使用AsOx / c-MWCNT / PANI / Au电极作为工作电极，Ag / AgCl（3 M /饱和KCl）作为标准电极，Pt线作为通过恒电位仪连接的辅助电极，制备了抗坏血酸生物传感器。线性范围，响应时间和检测极限分别为2-206μM，2 s和0.9μM。当在+0.6 V极化时，该生物传感器在pH 5.8和更宽的温度范围（30-45°C）中显示最佳响应。该生物传感器用于测定血清，果汁和维生素C片剂中的抗坏血酸水平。评估了传感器的血清中添加的抗坏血酸的回收率达到91％，五个血清样品的批变异系数之内和之间分别为6.5％和11.4％。通过标准的2,6-二氯苯酚吲哚酚（DCPIP）方法测得的果汁抗坏血酸值与本方法之间具有良好的相关性（r = 0.98）。当在4°C下保存时，酶电极在两个月内使用200次。与早期的酶传感器相比，该生物传感器的优势在于，由于酶与支持物的共价偶联，酶不泄漏，响应时间更短，工作范围更广，存储稳定性更高，并且不受血清物质的干扰。

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