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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes
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Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes

机译：基于蛋白质连接的金纳米粒子在丝网印刷电极上的选择性电沉积的Aptasensor

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摘要

The electrodeposition of DNA-gold nanoparticles previously developed in our group has been used as starting point for the electrodeposition of proteins attached to gold nanoparticles. We have performed a proof of principle by developing a methodology based on the electrodeposition of proteins bound to gold nanoparticles on screen-printed gold microelectrodes using, in a first approach, horseradish peroxide-conjugated gold nanoparticles (gold-HRP). The electrodeposition was achieved at a current positive potential of 800 mV vs. Ag/AgCl and the functionality of the electrodeposited HRP-particles was tested by electrochemical reduction of H_2O_2. Furthermore, we used this proof of concept in an aptasensor application to detect Leishmania infantum KMP-11. Hence, we have demonstrated not only the functionality of the electrodeposition of proteins bound to gold nanoparticles, but also the utility of the method with the aim of developing a real biosensor containing multiple enzymes or proteins in a multimodular device.
机译：我们小组中先前开发的DNA-金纳米颗粒的电沉积已用作电沉积附着在金纳米颗粒上的蛋白质的起点。我们已经通过开发一种方法来进行原理验证，该方法基于在第一种方法中使用辣根过氧化物共轭的金纳米颗粒（金HRP）在丝网印刷的金微电极上电沉积与金纳米颗粒结合的蛋白质。电沉积是在相对于Ag / AgCl的800 mV的正电势下实现的，通过H_2O_2的电化学还原测试了电沉积HRP粒子的功能。此外，我们在aptasensor应用程序中使用了这一概念验证来检测婴儿利什曼原虫KMP-11。因此，我们不仅证明了与金纳米颗粒结合的蛋白质的电沉积功能，而且还证明了该方法的实用性，目的是开发一种在多模块装置中包含多种酶或蛋白质的真实生物传感器。

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