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首页> 外文期刊>The European Journal of Neuroscience >The synaptic basis of disease
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The synaptic basis of disease

机译：疾病的突触基础

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Background: The accuracy of highly sensitive biomarker methods is often confounded by the presence of various circulating endogenous factors in samples causing matrix effects. Method: This article outlines two different biomarker methods: hepcidin enzyme-linked immunosorbent assay (ELISA) for which an orthogonal assessment of ELISA to liquid chromatography-tandem mass spectrometry was performed to examine the potential matrix effect, and sclerostin ELISA to evaluate the matrix effect. Results: Although the potential interfering effects of the endogenous hepcidin variants (prohepcidin and clipped) showed that these proteins had >30% immunoreactivity in ELISA, the hepcidin ELISA preferentially measures full-length hepcidin when the molar ratios of full-length to variants remain >1. The correlation of ELISA to liquid chromatography-tandem mass spectrometry results showed full-length hepcidin as the major form in diseased populations. Conclusion: A fit-for-for-purpose assessment of matrix effect/selectivity was also performed for each method. This article demonstrates the utility of a fit-for-purpose approach to assess the validity of biomarker methods in evaluating the interconnected parameters of matrix effects, sensitivity and selectivity.
机译：背景：高敏感度生物标志物方法的准确性通常会因样品中存在各种循环内源性因素而引起基质效应，从而造成混淆。方法：本文概述了两种不同的生物标志物方法：hepcidin酶联免疫吸附测定（ELISA），该方法对ELISA与液相色谱-串联质谱法进行了正交评估，以检测潜在的基质效应，而硬化蛋白ELISA用于评估基质效应。结果：尽管内源性铁调素变体（前铁调素和截短型）的潜在干扰作用表明这些蛋白质在ELISA中具有> 30％的免疫反应性，但当全长与变体的摩尔比保持> 1。 ELISA与液相色谱-串联质谱分析结果的相关性表明，全长铁调素是患病人群的主要形式。结论：每种方法还进行了适合目的的基质效应/选择性评估。本文展示了一种适合目的的方法，可用于评估生物标志物方法在评估基质效应，灵敏度和选择性的相互关联参数方面的有效性。

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