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首页> 外文期刊>The FEBS journal >Occurrence of glucosylsucrose [alpha-D-glucopyranosyl-(1 -> 2)-alpha-D-glucopyranosyl-(1 -> 2)-beta-D-fructofuranoside] and glucosylated homologues in cyanobacteria - Structural properties, cellular contents and possible function as thermoprotectants
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Occurrence of glucosylsucrose [alpha-D-glucopyranosyl-(1 -> 2)-alpha-D-glucopyranosyl-(1 -> 2)-beta-D-fructofuranoside] and glucosylated homologues in cyanobacteria - Structural properties, cellular contents and possible function as thermoprotectants

机译：蓝细菌中葡萄糖基蔗糖[α-D-吡喃葡萄糖基-（1-> 2）-α-D-吡喃葡萄糖基-（1-> 2）-β-D-果糖呋喃糖苷]和糖基化同源物的出现-结构性质，细胞含量和可能的功能作为热保护剂

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摘要

Little is known about the structure and function of oligosaccharides in cyanobacteria. In this study, a new class of saccharides from Nostoc was identified by MS and NMR techniques, consisting of alpha-d-glucopyranosyl-(1 -> 2)-[alpha-d-glucopyranosyl-(1 -> 2)](n)-beta-d-fructofuranosides ranging from the trisaccharide (n = 1) to decasaccharide (n = 8). In Nostoc ellipsosporum the cell content of saccharides increased 10-20-fold after heat stress (1 day, 40 degrees C) or during prolonged cultivation. Under these conditions the abundance of homologues of higher molecular mass (> pentasaccharide) increased and finally exceeded that of homologues of lower molecular mass including sucrose. Total intracellular content of the saccharides after heat stress was 5-10 mg.(g dry weight)(-1) corresponding to intracellular concentrations of 0.25-0.5% (w/v). A possible role of the oligosaccharides identified is in the protection of enzymes against heat inactivation. Whereas amylase from Nostoc was only weakly protected by the decasaccharide, alpha-amylase from porcine pancreas was more efficiently stabilized by the octasaccharide and decasaccharide. Evidence is presented for the widespread occurrence of the newly identified saccharides in cyanobacteria. The results are discussed including previous reports on cyanobacterial oligosaccharides and with respect to possible functions of these compounds in the living cell.
机译：关于蓝细菌中寡糖的结构和功能知之甚少。在这项研究中，通过MS和NMR技术鉴定了Nostoc中的一类新型糖类，其中包括α- d -吡喃葡萄糖基-（1-> 2）-[alpha- d -glucopyranosyl-（1-> 2）]（n）-beta- d -果糖呋喃糖苷的范围从三糖（n = 1）到十糖（n = 8）。在热胁迫（1天，40摄氏度）或长期培养期间，Nostoc ellipsosporum中糖类的细胞含量增加了10-20倍。在这些条件下，较高分子量的同系物（>五糖）的丰度增加，最终超过了包括蔗糖在内的较低分子量的同系物的丰度。热应激后糖的总细胞内含量为5-10 mg。（g干重）（-1），对应于0.25-0.5％（w / v）的细胞内浓度。所鉴定的寡糖的可能作用是保护酶免于热失活。来自Nostoc的淀粉酶仅受到十糖的弱保护，而来自猪胰腺的α-淀粉酶则被八糖和十糖更有效地稳定。提供了新发现的糖在蓝细菌中广泛发生的证据。讨论了结果，包括先前关于蓝藻寡糖的报道以及这些化合物在活细胞中的可能功能。

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