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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Development of New Methods to Introduce Unnatural Functional Molecules into Native Proteins for Protein Engineering
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Development of New Methods to Introduce Unnatural Functional Molecules into Native Proteins for Protein Engineering

机译：开发将非天然功能分子引入天然蛋白质进行蛋白质工程的新方法

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摘要

Proteins are one of the most sophisticated biomolecules that play important roles in many biological processes and recently have attracted much attention as a key molecular player in new fields of nanobio-science and -technology.To use protein molecules,suitable molecular interfaces for maintaining,regulating,and/or expanding the structure and activity of natural proteins are highly desirable.In addition,development of flexible strategies and new concepts to manipulate protein structures at precise atomic levels is an important challenge from the viewpoint of organic chemistry.In this account,we highlight two unique chemistry-based strategies,developed by our group,for incorporating unnatural molecules into natural proteins,which include (i) reconstitution of chemically modified cofactors with apo-proteins or -enzymes,and (ii) post-(photo)affinity labeling modification of natural proteins.Using these methods,various unnatural molecules were incorporated into natural proteins without any genetic manipulation to produce semi-artificial proteins,of which the function can be regulated,modulated,and/or read-out with help of the incorporated unnatural functionalities.
机译：蛋白质是最复杂的生物分子之一，在许多生物过程中都起着重要作用，最近作为纳米生物科学和技术新领域中的关键分子角色而受到了广泛关注。要使用蛋白质分子，合适的分子界面来维持，调节此外，从有机化学的角度出发，开发灵活的策略和在精确的原子水平上操纵蛋白质结构的新概念是一项重要的挑战。重点介绍了由我们小组开发的两种基于化学的独特策略，用于将非天然分子整合到天然蛋白质中，包括（i）用载脂蛋白或酶重建化学修饰的辅因子，以及（ii）（光）后亲和标记天然蛋白质的修饰。使用这些方法，可以将各种非天然分子掺入天然蛋白质而无需进行遗传操作以产生半人工蛋白质，其功能可以借助引入的非天然功能进行调节，调节和/或读出。

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