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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >High accumulation of legumin and Lea-like mRNAs during maturation is associated with increased conversion frequency of somatic embryos from pedunculate oak (Quercus robur L.)
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High accumulation of legumin and Lea-like mRNAs during maturation is associated with increased conversion frequency of somatic embryos from pedunculate oak (Quercus robur L.)

机译：成熟期间豆类和Lea样mRNA的高积累与带花梗橡木（Quercus robur L.）的体细胞胚转化频率增加有关

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摘要

The expression patterns of the storage protein legumin gene and Em- and dehydrin-like homologues were investigated in somatic embryos from Quercus robur L. The effect of different maturation treatments (1% agar, 6% sorbitol, or 5% polyethylene glycol) and partial desiccation on transcript accumulations as well as conversion capacity of somatic embryos was also investigated. Differential expression of putative Em- and two dehydrin-like homologues (designated as Dhn1a and Dhn1b) was detected in somatic oak embryos with heterologous probes. Low expression levels of legumin, Em- and dehydrin-like mRNAs were detected in somatic embryos prior to maturation treatment. A high accumulation of these transcripts was found in embryos that had been cultured on media supplemented with 6% sorbitol or 1% agar. These embryos also showed a high conversion frequency into plantlets. In contrast, no improvement in plant conversion as well as a low accumulation of legumin, Em-like and Dhn1b-like transcripts was observed in embryos that were matured on polyethylene glycol medium. Partial-desiccation treatment significantly enhanced the plant conversion. Nevertheless, a decline in expression of legumin, Em-like and Dhn1a-like homologues was detected upon dehydration. In contrast, Dhn1b and oak homologues to peach dehydrin were also strongly expressed in desiccated embryos. In addition , the treatment of embryos with abscisic acid promoted the accumulation of all investigated transcripts. These results suggest that the regulation of the legumin storage protein gene and Lea-like homologues in somatic oak embryos is under developmental control and that their regulation can be influenced by manipulating the culture conditions. [References: 37]
机译：研究了栎类体细胞胚中贮藏蛋白豆蛋白基因以及类似Em和脱水醇的同系物的表达模式。不同成熟方法（1％琼脂，6％山梨醇或5％聚乙二醇）和部分成熟处理的影响还研究了干燥对转录本积累以及体细胞胚转化能力的影响。使用异源探针在体橡树胚中检测到推定的Em和两个脱水蛋白样同源物（称为Dhn1a和Dhn1b）的差异表达。成熟处理之前，在体细胞胚中检测到豆类，Em和脱水素样mRNA的低表达水平。在补充了6％山梨醇或1％琼脂的培养基上培养的胚胎中发现了这些转录物的高积累。这些胚也显示出高转化成苗的频率。相反，在聚乙二醇培养基上成熟的胚中未观察到植物转化的改善以及豆类，Em样和Dhn1b样转录物的低积累。部分干燥处理显着提高了植物转化率。然而，脱水后检测到豆类，Em样和Dhn1a样同源物的表达下降。相反，Dhn1b和桃脱水蛋白的橡木同源物在干燥的胚中也强烈表达。另外，用脱落酸处理胚胎促进了所有研究转录本的积累。这些结果表明，豆科植物胚胚中豆类储藏蛋白基因和Lea样同源物的调控处于发育控制之下，其调控可受培养条件的影响。 [参考：37]

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