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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Spatio-temporal relationship between nuclear-envelope breakdown and preprophase band disappearance in cultured tobacco cells
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Spatio-temporal relationship between nuclear-envelope breakdown and preprophase band disappearance in cultured tobacco cells

机译：烟草细胞中核膜破裂与前期条带消失之间的时空关系

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Cell division involves the coordinated progression of karyokinesis and cytokinesis. which is accomplished by communication between the nucleus and the cytoplasm. We hake utilized green-fluorescent-protein technology to generate a line (A tobacco Bright Yellow 2' (BY-2) cells labeled for both microtubules and the nuclear envelope. This cell line allowed us to use living cells to investigate the relationship between nuclear-envelope breakdown and preprophase hand disappearance with high spatial and temporal resolution. Our observations demonstrate that nuclear-en elope breakdown always precedes preprophase band disappearance in BY-2 cells. In addition, the rate of preprophase band disappearance. and the attenuation of perinuclear microtubule fluorescence, correlates with the proximity of the nucleus to the preprophase band These results indicate the presence of communication between the nucleus and the preprophase band and suggest a causal relationship between nuclear-em elope breakdown and preprophase hand disappearance. [References: 20]
机译：细胞分裂涉及核运动和胞质分裂的协调发展。这是通过细胞核与细胞质之间的通讯来完成的。我们利用绿色荧光蛋白技术产生了一个细胞系（一种烟草的亮黄色2'（BY-2）细胞，被标记为微管和核膜。包膜破裂和前期手消失，具有很高的时空分辨率，我们的观察结果表明，核－肾小球破裂总是发生在BY-2细胞的前期频带消失之前，此外，前期频带消失的速率以及核周微管的衰减荧光，与细胞核与前期带的接近程度相关。这些结果表明核与前期带之间存在通信，并暗示核胚斜面破裂与前期手消失之间的因果关系[参考文献：20]。

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