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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Attachment of the parasitic weed dodder to the host
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Attachment of the parasitic weed dodder to the host

机译:将寄生性杂草d杂剂附着到宿主上

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The parasitic weed dodder (Cuscuta pentagona L.) invades a number of potential host species. but the mechanisms responsible for ensuring tight adhesion to the wide variety of host surfaces have yet to be identified. In this study, a battery of microscopy protocols is used to examine the host-parasite interface in an effort to deduce these mechanisms. As the dodder shoot approaches the host tissue, epidermal cells in the parasite shoot elongate and differentiate into secretory type trichomes. The trichome cell walls are malleable, allowing them to elongate towards the host and bend their walls to conform to the shape of the host cell surface, The presence of osmiophilic particles (probable cell-wall-loosening complexes) at far greater numbers than found in other species presages the expansion and malleable nature of the epidermal cells. In addition to the changes in cell shape, the dodder trichome cells secrete an electron-opaque cementing substance that covers the host-parasite interface. When probed with antibodies that recognize cell wall components, the cement reacted only with antibodies that recognize chiefly de-esterified pectins but not other common wall constituents. These data indicate that dodder utilizes both a cementing layer of pectin and a radically modified epidermal cell wall to secure the parasite to the perspective host. [References: 27]
机译:寄生杂草do丝草(Cuscuta pentagona L.)入侵许多潜在的寄主物种。但是尚未确定负责确保与多种宿主表面紧密粘附的机制。在这项研究中,使用了一系列的显微镜检查协议来检查宿主-寄生虫的界面,从而推断出这些机制。当the丝芽接近宿主组织时,寄生虫芽中的表皮细胞伸长并分化成分泌型毛状体。毛状细胞壁是可延展的,允许它们向宿主伸长并弯曲其壁以符合宿主细胞表面的形状。亲脂性颗粒(可能使细胞壁松弛的复合物)的存在数量远大于其他物种则预示着表皮细胞的膨胀和延展性。除细胞形状变化外,the子毛状体细胞还分泌一种电子不透明的胶结物质,该物质覆盖了宿主-寄生虫的界面。当用识别细胞壁成分的抗体探测时,骨水泥仅与主要识别去酯化果胶的抗体反应,而与其他常见的壁成分不识别。这些数据表明,do窃者既利用果胶的胶结层,又利用经自由基修饰的表皮细胞壁将寄生虫固定在透视宿主上。 [参考:27]

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