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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Temporal and subcellular localization of PR-1 proteins in tomato stem tissues infected by virulent and avirulent isolates of Phytophthora capsici
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Temporal and subcellular localization of PR-1 proteins in tomato stem tissues infected by virulent and avirulent isolates of Phytophthora capsici

机译：PR-1蛋白在辣椒干疫霉菌的有毒和无毒分离株感染下在番茄干组织中的时间和亚细胞定位

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摘要

Immunoblot analysis and immunogold labeling of PR-1 protein (pathogenesis-related protein 1) in tomato (Lycopersicon esculentum Mill.) were performed to examine the temporal and spatial expression patterns of PR-I protein induced by Phytophthora capsici infection. Soluble proteins with molecular masses of 10, 17,25,27 and 75 kDa were induced and accumulated in P capsici-infected stem tissues during the compatible and incompatible interactions. Western blot analysis revealed that expression of PR-1 protein (17 kDa), at 12 to 24 h after inoculation, occurred earlier in the incompatible than in the compatible interaction. Immunogold labeling of PR-1 proteins occurred over cell walls and cytoplasm of the host and the oomycete pathogen and at the interface between host and oomycete cell walls at 24 h after inoculation in the compatible interaction. In the incompatible interaction, numerous PR-1 proteins accumulated predominantly over oomycete cell walls and at the interface between host and oomycete cell walls. The quantity of PR-1 proteins deposited in both host and oomycete cells was much less in the compatible than the incompatible interaction. Healthy tomato stem tissue was nearly free of immunogold labeling of PR-1 proteins. [References: 41]
机译：进行了番茄（Lycopersicon esculentum Mill。）中PR-1蛋白（发病相关蛋白1）的免疫印迹分析和免疫金标记，以检查辣椒疫霉感染引起的PR-1蛋白的时空表达模式。在相容性和不相容性相互作用期间，分子质量分别为10、17、25、27和75 kDa的可溶性蛋白被诱导并积累在P辣椒感染的茎组织中。蛋白质印迹分析表明，PR-1蛋白（17 kDa）在接种后12至24 h的表达发生在不相容的发生时间早于相容的相互作用。 PR-1蛋白的免疫金标记发生在宿主与卵菌病原体的细胞壁和细胞质之间以及在相容相互作用中接种后24小时内，在宿主与卵菌细胞壁之间的界面处。在不相容的相互作用中，许多PR-1蛋白主要聚集在卵菌细胞壁上以及宿主和卵菌细胞壁之间的界面上。在宿主和卵菌细胞中沉积的PR-1蛋白的数量比不相容相互作用少得多。健康的番茄茎组织几乎没有PR-1蛋白的免疫金标记。 [参考：41]

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