Caged probes: a novel tool in studying symplasmic transport in plant tissues

Martens HJ; Hansen M; Schulz A

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Caged probes: a novel tool in studying symplasmic transport in plant tissues

机译：笼状探针：研究植物组织中同质转运的新型工具

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Caged probes offer a novel approach to study plant cell-to-cell communication. Instead of introducing fluorescent molecules into cells by microinjection, their caged counterparts can be preloaded into the tissue by diffusion. Following spatially controlled photoactivation, movement of the uncaged fluorochrome can be followed in time and direction by confocal laser scanning microscopy. In the onion bulb scale epidermis used as a model system, symplasmic transport of the tracer out of a target cell was followed. Transport via the symplasmic pathway was challenged by plasmolysing the tissue. The experiments confirmed the symplasmic nature of tracer transport.

机译：笼状探针提供了一种研究植物细胞间通讯的新颖方法。与其通过显微注射将荧光分子引入细胞中，还可以将它们的笼状对应物通过扩散预装到组织中。在空间控制的光活化之后，可以通过共聚焦激光扫描显微镜在时间和方向上跟踪未老化的荧光染料的运动。在用作模型系统的洋葱鳞茎鳞状表皮中，追踪示踪剂从靶细胞的同质转运。通过浆膜裂解组织挑战了通过同质途径的运输。实验证实了示踪剂运输的同质性。

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《Protoplasma: An International Journal of Cell Biology》 |2004年第1期|共4页
作者
Martens HJ; Hansen M; Schulz A;
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正文语种 eng
中图分类细胞形态学;
关键词
caged fluorescein; intercellular communication; onion bulb epidermis; Allium cepa; photoactivation; plasmodesma; LIVING CELLS; FREE CALCIUM; PLASMODESMATA; PHOTOLYSIS; MOVEMENT; CLOSURE;

机译：笼状荧光素;细胞间通讯;洋葱鳞茎表皮;葱属;光活化;血浆;活细胞;无钙;等离子;光解;运动;关闭;

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1. Caged probes: a novel tool in studying symplasmic transport in plant tissues [J] . H. J. Martens, M. Hansen, A. Schulz Protoplasma . 2004,第1期

机译：笼状探针：研究植物组织内同质转运的新工具
2. Protein toxins from plants and bacteria: probes for intracellular transport and tools in medicine. [J] . Sandvig K, Torgersen ML, Engedal N, FEBS letters. . 2010,第12期

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3. Protein toxins from plants and bacteria: Probes for intracellular transport and tools in medicine [J] . FEBS Letters . 2010,第12期

机译：来自植物和细菌的蛋白质毒素：细胞内转运的探针和医学工具
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5. Fatty acid metabolism in higher plants: Probing the mechanism of fatty acid transport across the chloroplast envelope. [D] . Koo, Abraham Jeong-Kyu. 2004

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7. Protein toxins from plants and bacteria: Probes for intracellular transport and tools in medicine [O] . Sandvig Kirsten, Torgersen Maria L., Engedal Nikolai, 2010

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8. Monte Carlo simulations incorporating Mie calculations of light transport in tissue phantoms: Examination of photon sampling volumes for endoscopically compatible fiber optic probes [R] . Mourant, J. R. , Hielscher, A. H. , Bigio, I. J. 1996

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Caged probes: a novel tool in studying symplasmic transport in plant tissues

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