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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Regeneration of lipophilic antioxidants by NAD(P)H : quinone oxidoreductase 1
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Regeneration of lipophilic antioxidants by NAD(P)H : quinone oxidoreductase 1

机译：NAD（P）H：醌氧化还原酶1再生亲脂性抗氧化剂

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The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase I designated as "hydrophilic" and "hydrophobic" (H. J. Prochaska and P. Talalay. Journal of Biological Chemistry 261: 1372-1378,1986) show differential enzyme activities towards hydrophilic or hydrophobic ubiquinone homologs. By chromatography on phenyl Sepharose, we purified the two isoforms from pig liver cytosol and measured their reduction of several ubiquinone homologs of different side chain length. We also studied by electron paramagnetic resonance the effect of NAD(P)H:(quinone acceptor) oxidoreductase 1 on steady-state levels of chromanoxyl radicals generated by linoleic acid and lipooxygenase and confirmed the enzyme's ability to protect alpha-tocopherol against oxidation induced with H2O2-Fe2+. Our results demonstrated that the different hydrophobicities of the isoforms do not reflect different reactivities towards ubiquinones of different side chain length. In addition, electron paramagnetic resonance studies showed that in systems containing the reductase plus NADH, levels of chromanoxyl radicals were dramatically reduced. Morever, in the presence of oxidants, alpha-tocopherol was preserved by NAD(P)H:(quinone acceptor) oxidoreductase 1, supporting our hyothesis that regeneration of alpha-tocopherol may be one of the physiologic functions of this enzyme. [References: 29]
机译：这项工作的目的是研究NAD（P）H :(醌受体）氧化还原酶1（EC 1.6.99.2）在亲脂性抗氧化剂，α-生育酚和还原型辅酶Q类似物再生中的活性。首先，我们测试了NAD（P）H :(醌受体）氧化还原酶I的两个同工型是否称为“亲水性”和“疏水性”（HJ Prochaska和P. Talalay。Journal of Biological Chemistry 261：1372-1378， 1986）显示出不同的酶活性对亲水或疏水泛醌同系物。通过色谱法在苯基琼脂糖凝胶上，我们从猪肝细胞溶胶中纯化了两种同工型，并测量了它们对不同侧链长度的几种泛醌同系物的减少。我们还通过电子顺磁共振研究了NAD（P）H :(醌受体）氧化还原酶1对亚油酸和脂氧加合酶生成的苯并氧自由基的稳态水平的影响，并证实了该酶具有保护α-生育酚抵抗由H诱导的氧化的能力。 H2O2-Fe2 +。我们的结果表明，同工型的不同疏水性不能反映对不同侧链长度的泛醌的不同反应性。此外，电子顺磁共振研究表明，在包含还原酶和NADH的系统中，苯并吡喃氧基的水平显着降低。此外，在存在氧化剂的情况下，NAD（P）H :(醌受体）氧化还原酶1可以保护α-生育酚，这支持了我们的假设：α-生育酚的再生可能是该酶的生理功能之一。 [参考：29]

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