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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Possible involvement of energy metabolism in the change of cytoplasm organization induced by a protein phosphatase inhibitor, calyculin A, in root hair cells of Limnobium stoloniferum
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Possible involvement of energy metabolism in the change of cytoplasm organization induced by a protein phosphatase inhibitor, calyculin A, in root hair cells of Limnobium stoloniferum

机译：能量的代谢可能参与了由植物磷酸根（Limnobium stoloniferum）根毛细胞中的一种蛋白磷酸酶抑制剂（calyculin A）诱导的细胞质组织变化。

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In root hair cells of Limnobium stoloniferum, transvacuolar strands disperse and cytoplasmic spherical bodies (CSBs) emerge upon treatment with a protein phosphatase inhibitor, calyculin A (CA), whose effects were previously shown to be canceled by simultaneous treatment of the cells with a nonselective protein kinase inhibitor, K-252a. CSB formation is also suppressed by latrunculin B (LB) or cytochalasin D, actin filament depolymerization drugs, or 2,3-butanedione monoxime, an inhibitor of myosin activity. To confirm the involvement of myosin activity in CSB formation induced by CA, we examined the effect of an inhibitor of energy metabolism, NaN3, on CSB formation in root hair cells pretreated simultaneously with CA and LB. In the presence of CA-LB, CSB formation was suppressed due to the depolymerization of actin filaments. When these drugs were removed, the actin filaments recovered and CSBs emerged even in the presence of K-252a. These results indicated that the phosphorylation level in the cells is elevated during the CA-LB treatment and that a phosphorylation level sufficient for the CSB formation was sustained even after CA removal. On the other hand, CSB formation after simultaneous treatment with CA and LB was significantly suppressed in the presence of NaN3. In such cells, actin filament bundles recovered, although their organization was random. The present and previous results suggested that myosin activity is necessary for CSB formation induced by CA, and that myosin regulated by phosphorylation-dephosphorylation is implicated in the organization of the actin cytoskeleton in root hair cells. [References: 44]
机译：在stomniferium stoloniferum的根毛细胞中，经蛋白磷酸酶抑制剂calyculin A（CA）处理后，透真空链散布并出现胞质球形体（CSB），以前证明通过同时用非选择性细胞同时处理可消除其作用蛋白激酶抑制剂，K-252a。 latrunculin B（LB）或细胞松弛素D，肌动蛋白丝解聚药物或肌球蛋白活性抑制剂2,3-丁二酮一肟也可抑制CSB的形成。为了确认肌球蛋白活性参与由CA诱导的CSB形成，我们检查了能量代谢抑制剂NaN3对同时用CA和LB预处理的根毛细胞中CSB形成的影响。在CA-LB的存在下，由于肌动蛋白丝的解聚，抑制了CSB的形成。除去这些药物后，肌动蛋白丝恢复原状，即使在K-252a存在的情况下也出现了CSB。这些结果表明，在CA-LB处理过程中细胞中的磷酸化水平升高，并且即使去除CA后，仍足以维持CSB形成的磷酸化水平。另一方面，在存在NaN 3的情况下，用CA和LB同时处理后CSB的形成被显着抑制。在这种细胞中，肌动蛋白丝束恢复了，尽管它们的组织是随机的。目前和以前的结果表明，肌球蛋白活性对于由CA诱导的CSB形成是必需的，并且由磷酸化-去磷酸化调节的肌球蛋白与根毛细胞中肌动蛋白细胞骨架的组织有关。 [参考：44]

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