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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Iron deficiency enhances the levels of ascorbate, glutathione, and related enzymes in sugar beet roots
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Iron deficiency enhances the levels of ascorbate, glutathione, and related enzymes in sugar beet roots

机译：缺铁会提高甜菜根中抗坏血酸，谷胱甘肽和相关酶的水平

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摘要

The effects of Fe deficiency stress on the levels of ascorbate and glutathione, and on the activities of the enzymes ferric chelate reductase, glutathione reductase (EC 1.6.4.2), ascorbate free-radical reductase (EC 1.6.5.4) and ascorbate peroxidase (EC 1.11.1.11), have been investigated in sugar beet (Beta vulgaris L.) roots. Plasma membrane vesicles and cytosolic fractions were isolated from the roots of the plants grown in nutrient solutions in the absence or presence of Fe for two weeks. Plants responded to Fe deficiency not only with a 20-fold increase in root ferric chelate reductase activity, but also with moderately increased levels of the general reductants ascorbate (2-fold) and glutathione (1.6-fold). The enzymes of the ascorbate-glutathione cycle in roots were also affected by Fe deficiency. Glutathione reductase activity was enhanced 1.4-fold with Fe deficiency, associated to an increased ratio of reduced to oxidized glutathione, from 3.1 to 5.2. The plasma membrane fraction from iron-deficient roots showed 1.7-fold higher ascorbate free-radical reductase activity, whereas in the cytosolic fraction the enzyme activity was not affected by Fe deficiency. The activity of the cytosolic hemoprotein ascorbate peroxidase decreased approximately by 50% with Fe deprivation. These results show that sugar beet responds to Fe deficiency with metabolic changes affecting components of the ascorbate-glutathione cycle in root cells. This suggests that the ascorbate-glutathione cycle would play certain roles in the general Fe deficiency stress responses in strategy I plants. [References: 40]
机译：铁缺乏胁迫对抗坏血酸和谷胱甘肽水平以及铁螯合还原酶，谷胱甘肽还原酶（EC 1.6.4.2），抗坏血酸自由基还原酶（EC 1.6.5.4）和抗坏血酸过氧化物酶（EC）活性的影响1.11.1.11），已经在甜菜（Beta vulgaris L.）根中进行了研究。从在营养溶液中生长的植物的根中，在不存在或不存在铁的情况下，分离两周的质膜囊泡和胞质部分。植物对铁缺乏的反应不仅使根中的铁螯合物还原酶活性增加了20倍，而且使一般还原剂抗坏血酸（2倍）和谷胱甘肽（1.6倍）的水平适度增加。根中抗坏血酸-谷胱甘肽循环的酶也受到铁缺乏的影响。谷胱甘肽还原酶活性因铁缺乏而提高了1.4倍，这与还原型谷胱甘肽与氧化型谷胱甘肽的还原比例从3.1增加到5.2有关。铁缺乏根的质膜部分显示出高1.7倍的抗坏血酸自由基还原酶活性，而在胞质部分中，酶活性不受铁缺乏的影响。随着铁的剥夺，胞质血蛋白抗坏血酸过氧化物酶的活性降低了约50％。这些结果表明，甜菜对铁缺乏有响应，其代谢变化会影响根细胞中抗坏血酸-谷胱甘肽循环的成分。这表明抗坏血酸-谷胱甘肽循环将在策略I植物的一般铁缺乏胁迫响应中发挥某些作用。 [参考：40]

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