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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Strict paternal transmission of mitochondrial DNA of Chlamydomonas species is explained by selection against maternal nucleoids
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Strict paternal transmission of mitochondrial DNA of Chlamydomonas species is explained by selection against maternal nucleoids

机译：衣原体物种线粒体DNA的严格父系传播是通过针对母体核苷酸的选择来解释的

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摘要

The non-Mendelian inheritance of organelle DNA is common in most plants and animals. Here we examined inheritance mechanisms involved in the transfer of mitochondrial DNA. We successively backcrossed (to F-5) two interfertile strains of the unicellular isogamous haploid algae Chlamydomonas reinhardtii and Chlamydomonas smithii to match nuclear backgrounds and examine transmission patterns of mitochondrial DNA by PCR analysis of cob gene sequences. Mitochondrial DNA was strictly transmitted paternally. To investigate the behavior of parental mitochondrial DNA, we used F-5 progeny to form zygotes and isolated single zygotes. The results showed selective disappearance of maternal mitochondrial nucleoids occurred between 3 and 6 h after zygote formation. [References: 24]
机译：细胞器DNA的非孟德尔遗传是常见于大多数动植物中。在这里，我们检查了涉及线粒体DNA转移的遗传机制。我们连续回交（至F-5）单细胞同配子体单倍体藻类莱茵衣藻和史密斯衣藻的两个可育菌株以匹配核背景，并通过PCR分析cob基因序列检查线粒体DNA的传播方式。线粒体DNA严格通过父系传播。为了研究亲代线粒体DNA的行为，我们使用F-5后代形成合子和分离出的单个合子。结果表明，合子形成后3至6小时内，母体线粒体核苷酸选择性消失。 [参考：24]

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