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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Changes in the centrin and microtubule cytoskeletons after metaphase arrest of the Chlamydomonas reinhardtii met1 mutant
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Changes in the centrin and microtubule cytoskeletons after metaphase arrest of the Chlamydomonas reinhardtii met1 mutant

机译：莱茵衣藻met1突变体中期停滞后中心蛋白和微管细胞骨架的变化

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摘要

The temperature-sensitive conditional met1 Chlamydomonas reinhardtii mutant arrests in metaphase at the restrictive temperature (33degreesC) with an intact spindle and high cell division kinase levels. In this study, met1 was investigated with respect to changes in the microtubule and centrin-based cytoskeletons after arrest at 33degreesC. Immunofluorescence microscopy revealed that, initially on arrest, the microtubule spindle and centrin-based cytoskeleton appeared as previously reported for wild-type metaphase cells; crescent-shaped spindles were seen with two brightly labelled centrin foci at each spindle pole in the basal body region at the cell surface. Observation of met1 held at the restrictive temperature reveals spindles can detach from one spindle pole and chromosomes eventually detach from the spindles. Moreover, a pseudo-anaphase event of spindle and nucleus elongation occurs in the absence of chromosome separation. Electron microscopy confirms that cytokinesis is initiated, the nuclei maintain a crescent shape but are distended and multiple pyrenoids are detected, suggesting chloroplast division also continues. Interestingly, prolamellar-like bodies usually present in etioplasts of dark-grown plants appear at the nuclear envelope. These results are discussed in relation to the coordination of division events in Chlamydomonas reinhardtii and the loss of viability in arrested cells of this mutant.
机译：温度敏感的条件性met1衣藻衣原体突变体在完整的纺锤体和高细胞分裂激酶水平的限制性温度（33℃）下在中期停滞。在这项研究中，调查了met1在33°C停滞后微管和基于蛋白的细胞骨架的变化。免疫荧光显微镜显示，最初被捕时，微管纺锤体和基于蛋白的细胞骨架出现，如先前报道的野生型中期细胞一样。看到新月形的纺锤体，在细胞表面的基体区域的每个纺锤体极上都有两个明亮标记的中心蛋白焦点。观察在限定温度下保存的met1发现纺锤体可以从一个纺锤体极上脱离，而染色体最终从纺锤体上脱离。此外，在缺乏染色体分离的情况下发生纺锤体和核伸长的假后期事件。电子显微镜确认胞质分裂开始，细胞核保持新月形，但被扩张并检测到多个类胡萝卜素，表明叶绿体分裂也继续进行。有趣的是，通常存在于深色植物的原生质体中的prolamellar状体出现在核被膜处。讨论了与莱茵衣藻中的分裂事件的协调和该突变体的滞留细胞的活力丧失有关的这些结果。

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