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Morphological and quantitative data of plastids and mitochondria within drought-stressed spinach leaves

机译:干旱胁迫下菠菜叶中质体和线粒体的形态学和定量数据

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Selected cell organelles were investigated at a high level of resolution with the transmission electron microscope, using ultrathin serial sections to create three-dimensional reconstructions. On the basis of these reconstructions. morphological data of chloroplast fine structures, mitochondria. and peroxisomes from control and drought-stressed spinach leaves were evaluated and compared. Mesophyll cell chloroplasts of control plants contained 60% stroma, 23% thylakoids, and 16% starch. In drought-stressed plants. the volume of both the stroma and the thylakoids increased to 68% and 32%, respectively. The amount of plastoglobuli was about 0.3% in both samples. Chloroplasts of stressed plants differed from control plants not only in the thylakoid and stroma values but also in the lack of starch grains. Mitochondria occurred in variable forms in control and stressed samples. In stressed plants, mitochondria had only 65% of the volume compared with control plants. Peroxisomes were inconspicuous.
机译:使用透射电子显微镜,使用超薄系列切片创建三维重建物,以高分辨率对选定的细胞器进行了研究。在这些重建的基础上。叶绿体细结构,线粒体的形态学数据。对对照和干旱胁迫的菠菜叶片中的过氧化物酶体和过氧化物酶体进行了评估和比较。对照植物的叶肉细胞叶绿体含有60%的基质,23%的类囊体和16%的淀粉。在干旱胁迫的植物中。基质和类囊体的体积分别增加到68%和32%。在两个样品中,质体球的含量约为0.3%。胁迫植物的叶绿体不仅在类囊体和基质值方面而且在缺乏淀粉粒方面与对照植物不同。线粒体以可变形式出现在对照和压力样品中。与对照植物相比,在受胁迫的植物中,线粒体仅占体积的65%。过氧化物酶体不明显。

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