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Profilin and Rop GTPases are localized at infection sites of plant cells

机译:Profilin和Rop GTPases定位在植物细胞的感染部位

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We have found 5 profilin cDNAs in cultured parsley cells, representing a small gene family of about 5 members in parsley. Specific antibodies were produced using heterologously expressed parsley profilin as antigen. Western blot analysis revealed the occurrence of similar amounts of profilin in roots and green parts of parsley plants. Immunocytochemical staining of parsley cells infected with the oomycetous plant pathogen Phytophthora infestans clearly revealed that profilin accumulates at the site on the plasma membrane subtending the oomycetous appressorium, where the actin cables focus. We also observed the accumulation of Rop GTPases around this site, which might point to a potential function in signaling to the cytoskeleton.
机译:我们在培养的欧芹细胞中发现了5个profilin cDNA,代表欧芹中约​​5个成员的小基因家族。使用异源表达的欧芹前体蛋白作为抗原产生特异性抗体。蛋白质印迹分析表明,在香菜植物的根部和绿色部分中出现了相似量的profilin。感染了卵菌性植物病原菌疫霉菌的欧芹细胞的免疫细胞化学染色清楚地表明,profilin聚集在质膜上,紧贴卵菌性前质膜,是肌动蛋白电缆集中的部位。我们还观察到了Rop GTPases在该位点附近的积累,这可能表明在向细胞骨架发出信号方面的潜在功能。

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