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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Possible association of actin filaments with chloroplasts of spinach mesophyll cells in vivo and in vitro
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Possible association of actin filaments with chloroplasts of spinach mesophyll cells in vivo and in vitro

机译：肌动蛋白丝与菠菜叶肉细胞叶绿体在体内和体外的可能联系

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In palisade mesophyll cells of spinach (Spinacia oleracea L.) kept under low-intensity white light, chloroplasts were apparently immobile and seemed to be surrounded by fine bundles of actin filaments. High-intensity blue light induced actin-dependent chloroplast movement concomitant with the appearance of a couple of long, straight bundles of actin filaments in each cell, whereas high-intensity red light was essentially ineffective in inducing these responses. The actin organization observed under low-intensity white light has been postulated to function in anchoring chloroplasts at proper intracellular positions through direct interaction with the chloroplasts. Intact chloroplasts, which retained their outer envelopes, were isolated after homogenization of leaves and Percoll centrifugation. No endogenous actin was detected by immunoblotting in the final intact-chloroplast fraction prepared from the leaves kept under low-intensity white light or in darkness. In cosedimentation assays with exogenously added skeletal muscle filamentous actin, however, actin was detected in the intact-chloroplast fraction precipitated after low-speed centrifugation. The association of actin with chloroplasts was apparently dependent on incubation time and chloroplast density. After partial disruption of the outer envelope of isolated chloroplasts by treatment with trypsin, actin was no longer coprecipitated. The results suggest that chloroplasts in spinach leaves can directly interact with actin, and that this interaction may be involved in the regulation of intracellular positioning of chloroplasts.
机译：在低强度白光下菠菜的栅栏叶肉细胞（Spinacia oleracea L.）中，叶绿体显然不动，似乎被肌动蛋白丝的细束包围。高强度蓝光诱导肌动蛋白依赖性的叶绿体运动，并伴随着每个细胞中几对肌动蛋白丝的长直束的出现，而高强度红光在诱导这些反应方面基本上无效。据推测，在低强度白光下观察到的肌动蛋白组织通过与叶绿体的直接相互作用，将叶绿体锚定在适当的细胞内位置。在叶片均质化和Percoll离心分离后，保留保留其外壳的完整叶绿体。在低强度白光或黑暗条件下，从叶片制备的最终完整叶绿体级分中，通过免疫印迹未检测到内源性肌动蛋白。然而，在外源添加骨骼肌丝状肌动蛋白的共沉淀试验中，在低速离心后沉淀的完整叶绿体部分中检测到肌动蛋白。肌动蛋白与叶绿体的关联显然取决于孵育时间和叶绿体密度。用胰蛋白酶处理部分破坏了分离的叶绿体的外壳后，肌动蛋白不再共沉淀。结果表明菠菜叶中的叶绿体可以直接与肌动蛋白相互作用，并且这种相互作用可能参与叶绿体细胞内定位的调节。

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