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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >In vitro microspore selection in maize anther culture with oxidative-stress stimulators
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In vitro microspore selection in maize anther culture with oxidative-stress stimulators

机译：氧化应激刺激剂在玉米花药培养中的体外小孢子选择

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摘要

In order to produce doubled-haploid maize plants tolerant of oxidative stress, in vitro microspore selection was carried out in anther culture with reactive oxygen species (ROS) progenitors such as paraquat, menadione, tert-butylhydroperoxide (t-BHP), and methionine combined with riboflavin. All the ROS progenitors reduced the anther induction, the formation of microspore-derived structures, and their regeneration potential. Abnormal cell divisions and progeny cell degradation could be observed during the development of microspores treated with ROS progenitors. Menadione and t-BHP influenced the microspore developmental pathway, as menadione induced the formation of embryoids, while t-BHP increased the proportion of calli in the microspore-derived structures. As the result of in vitro selection, 15, 10, 10, and 3 fertile doubled-haploid plants were obtained in cultures treated with paraquat, t-BHP, methionine combined with riboflavin, and menadione, respectively.
机译：为了生产能耐受氧化应激的双倍体单倍体玉米植物，在花药培养中用活性氧物种（ROS）祖细胞（如百草枯，甲萘醌，叔丁基过氧化氢（t-BHP）和蛋氨酸结合）进行了体外小孢子选择。与核黄素。所有的ROS祖细胞都减少了花药的诱导，小孢子衍生结构的形成以及它们的再生潜力。在用ROS祖细胞处理的小孢子发育过程中，可以观察到异常的细胞分裂和后代细胞降解。甲萘醌和t-BHP影响小孢子的发育途径，因为甲萘醌诱导胚状体的形成，而t-BHP增加了小孢子衍生结构中愈伤组织的比例。作为体外选择的结果，在分别用百草枯，t-BHP，蛋氨酸与核黄素和甲萘醌处理的培养物中获得了15、10、10和3棵可育的双单倍体植物。

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