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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >A class Vb chitin synthase in Colletotrichum graminicola is localized in the growing tips of multiple cell types, in nascent septa, and during septum conversion to an end wall after hyphal breakage
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A class Vb chitin synthase in Colletotrichum graminicola is localized in the growing tips of multiple cell types, in nascent septa, and during septum conversion to an end wall after hyphal breakage

机译：炭疽菌中的Vb类几丁质合酶位于多种细胞类型的生长尖端，新生隔片中以及在菌丝破裂后隔片转化为端壁的过程中

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摘要

Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We complemented a chsA null mutant with chsA fused to the green-fluorescent protein (sgfp) gene driven by a heterologous constitutively expressed promoter. Comparisons of the strain with the ectopic chsA-sgfp to the wild type indicated that ChsA-sGFP serves the same biological functions as ChsA in that like the wild type, the chsA Delta chsA::sgfp (EC) had conidia that did not explode and hyphal tips that did not swell. Confocal microscopy of ChsA-sGFP (EC) cells stained with the membrane stain FM 4-64 (N-(3-triethylammoniumpropyl)-4-(6(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide) indicated that ChsA is localized in the plasma membrane of the following: growing apices of hyphal branches, conidiophores, and falcate and oval conidia; in nascent septa; and in septa that are being converted to an end wall after byphal breakage. The data support the hypothesis that chsA either directly or indirectly encodes the information for its localization, that ChsA is localized in the plasma membrane, and that the class Vb enzyme produces chitin synthase in multiple cells and after wall breakage.
机译：以前对几丁质合酶Vb类无效突变体（Colletotrichum graminicola chsA）的补充表明，编码的蛋白质约占分生甲壳质的30％，对于具有高水势的培养基中的分生孢子壁强度至关重要，并有助于增强菌丝强度提示。我们用融合到由异源组成性表达启动子驱动的绿色荧光蛋白（sgfp）基因的chsA补充了chsA空突变体。将异位chsA-sgfp菌株与野生型进行比较表明，ChsA-sGFP具有与ChsA相同的生物学功能，就像野生型一样，chsA Delta chsA :: sgfp（EC）的分生孢子没有爆炸，菌丝尖端没有肿胀。用膜染色剂FM 4-64（N-（3-三乙基铵丙基）-4-（6（4-（二乙基氨基）苯基）己三烯基）二溴化吡啶）染色的ChsA-sGFP（EC）细胞的共聚焦显微镜检查在以下的质膜中：菌丝分支，分生孢子以及镰状和卵形分生孢子的生长的顶端;在新生隔中并且在隔隔中，在ph骨断裂后将其转换为端壁。数据支持以下假设：chsA直接或间接编码其定位信息，ChsA定位在质膜中，Vb类酶在多个细胞中以及壁破裂后产生几丁质合酶。

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