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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Plasmolysis and recovery of different cell types in cryoprotected shoot tips of Mentha X piperita
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Plasmolysis and recovery of different cell types in cryoprotected shoot tips of Mentha X piperita

机译：薄荷（Mentha X piperita）的防冻梢中的不同细胞类型的溶质和恢复

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摘要

Successful cryopreservation of plant shoot tips is dependent upon effective desiccation through osmotic or physical processes. Microscopy techniques were used to determine the extent of cellular damage and plasmolysis that occurs in peppermint (Mentha x piperita) shoot tips during the process of cryopreservation, using the cryoprotectant plant vitrification solution 2 (PVS2) (30% glycerol, 15% dimethyl sulfoxide, 15% ethylene glycol, 0.4 M sucrose) prior to liquid-nitrogen exposure. The meristem cells were the smallest and least plasmolyzed cell type of the shoot tips, while the large, older leaf and lower cortex cells were the most damaged. When treated with cryoprotectant solutions, meristem cells exhibited concave plasmolysis, suggesting that this cell type has a highly viscous protoplasm, and protoplasts have many cell wall attachment sites. Shoot tip cells were most severely plasmolyzed after PVS2 treatment, liquid-nitrogen exposure, and warming in 1.2 M sucrose. Successful recovery may be dependent upon surviving the plasmolytic conditions induced by warming and diluting treated shoot tips in 1.2 M sucrose solutions. In peppermint shoot tips, clumps of young meristem or young leaf cells survive the cryopreservation process and regenerate plants containing many shoots. Cryoprotective treatments that favor survival of small, meristematic cells and young leaf cells are most likely to produce high survival rates after liquid-nitrogen exposure.
机译：植物枝梢的成功冷冻保存取决于通过渗透或物理过程的有效干燥。使用冷冻保护剂植物玻璃化溶液2（PVS2）（30％的甘油，15％的二甲亚砜，在暴露液氮之前，先加入15％乙二醇，0.4 M蔗糖）。分生组织细胞是茎尖中最小的和最少的胞浆裂解细胞类型，而较大的，较老的叶片和较低的皮层细胞受到的损害最大。当用冷冻保护剂溶液处理时，分生组织细胞表现出凹的溶质，表明该细胞类型具有高粘性的原生质，原生质体具有许多细胞壁附着位点。在PVS2处理，液氮暴露并在1.2 M蔗糖中加热后，茎尖细胞的胞质溶解最严重。成功的恢复可能取决于能否通过在1.2 M蔗糖溶液中加热和稀释处理后的芽梢而导致的溶浆条件是否得以维持。在薄荷芽梢中，成年的分生组织或年轻的叶细胞团在冷冻保存过程中存活下来，并再生出含有许多芽的植物。有利于小的分生组织细胞和幼叶细胞存活的低温保护疗法最有可能在暴露液氮后产生较高的存活率。

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