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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Recent progress in living cell imaging of plant cytoskeleton and vacuole using fluorescent-protein transgenic lines and three-dimensional imaging
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Recent progress in living cell imaging of plant cytoskeleton and vacuole using fluorescent-protein transgenic lines and three-dimensional imaging

机译：荧光蛋白转基因株系和三维成像在植物细胞骨架和液泡活细胞成像中的最新进展

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In higher-plant cells, microtubules, actin microfilaments, and vacuoles play important roles in a variety of cellular events, including cell division, morphogenesis, and cell differentiation. These intracellular structures undergo dynamic changes in their shapes and functions during cell division and differentiation, and to analyse these sequential structural changes, the vital labelling technique, using the green-fluorescent protein or other fluorescent proteins, has commonly been used to follow the localisation and translocation of specific proteins. To visualise microtubules, actin filaments, and vacuoles, several strategies are available for selecting the appropriate fluorescent-protein fusion partner: microtubule-binding proteins, tubulin, and plus-end-tracking proteins are most suitable for microtubule labelling; the actin binding domain of mouse talin and plant fimbrin for actin microfilament visualisation; and the tonoplast-intrinsic proteins and syntaxin-related proteins for vacuolar imaging. In addition, three-dimensional reconstruction methods are indispensable for localising the widely distributed organelles within the cell. The maximum intensity projection method is suitable for cytoskeletal structures, while contour-based surface modelling possesses many advantages for vacuolar membranes. In this article, we summarise the recent progress in living cell imaging of the plant cytoskeleton and vacuoles using various fusions with green-fluorescent proteins and three-dimensional imaging techniques.
机译：在高等植物细胞中，微管，肌动蛋白微丝和液泡在多种细胞事件中起重要作用，包括细胞分裂，形态发生和细胞分化。这些细胞内结构在细胞分裂和分化过程中会经历形状和功能的动态变化，为了分析这些顺序的结构变化，使用绿色荧光蛋白或其他荧光蛋白的重要标记技术通常被用来跟踪定位和特定蛋白质的易位。为了可视化微管，肌动蛋白丝和液泡，有几种策略可供选择，以选择合适的荧光蛋白融合伴侣：微管结合蛋白，微管蛋白和正向追踪蛋白最适合微管标记;小鼠塔林蛋白和植物纤维蛋白的肌动蛋白结合域，用于肌动蛋白微丝的可视化;液泡成像的液泡膜内在蛋白和语法相关蛋白。另外，三维重建方法对于定位细胞内广泛分布的细胞器是必不可少的。最大强度投影法适用于细胞骨架结构，而基于轮廓的表面建模对于液泡膜具有许多优势。在本文中，我们总结了使用各种与绿色荧光蛋白融合的蛋白质和三维成像技术对植物细胞骨架和液泡进行活细胞成像的最新进展。

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