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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cardosins in postembryonic development of cardoon: towards an elucidation of the biological function of plant aspartic proteinases
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Cardosins in postembryonic development of cardoon: towards an elucidation of the biological function of plant aspartic proteinases

机译：菜豆胚后胚发育过程中的心果蛋白酶：阐明植物天冬氨酸蛋白酶的生物学功能

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摘要

Following on from previous work, the temporal and spatial accumulation of the aspartic proteinases (EC 3.4.23) cardosin A and cardosin B during postembryonic seed development of cardoon (Cynara cardunculus) was studied. mRNA and protein analyses of both cardosins suggested that the proteins accumulate during seed maturation, and that cardosin A is later synthesised de novo at the time of radicle emergence. Immunocytochemistry revealed that the precursor form of cardosin A accumulates in protein bodies and cell walls. This localisation in seeds is different from that previously described for cardoon flowers, suggesting a tissue-dependent targeting of the protein. It is known that procardosins are active and may have a role in proteolysis and processing of storage proteins. However, the presence of procardosin A in seeds could be related to the proposed role of the plant-specific insert in membrane lipid conversion during water uptake and solute leakage in actively growing tissues. This is in accordance with the recently proposed bifunctional role of aspartic proteinase precursor molecules that possess a membrane-destabilising domain in addition to a protease domain. Mature cardosin B, but not its mRNA, was detected in the first hours after seed imbibition and disappeared at the time of radicle emergence. This extracellular aspartic protease has already been implicated in cell wall loosening and remodelling, and its role in seed germination could be related to loosening tissue constraints for radicle protusion. The described pattern of cardosin A and B expression suggests a finely tuned developmental regulation and prompts an analysis of their possible roles in the physiology of postembryonic development.
机译：在先前工作的基础上，研究了在菜豆（Cynara cardunculus）的胚后种子发育过程中天冬氨酸蛋白酶（EC 3.4.23）cardosin A和cardosin B的时间和空间积累。两种卡多菌素的mRNA和蛋白质分析表明，蛋白质在种子成熟过程中积累，而卡多菌素A后来在胚根出现时从头合成。免疫细胞化学显示，心蛋白酶A的前体形式积累在蛋白质体和细胞壁中。种子中的这种定位不同于先前对桔梗花的描述，表明该蛋白质的组织依赖性靶向。已知心蛋白酶原是有活性的，并且可能在蛋白水解和储存蛋白的加工中起作用。但是，种子中原心蛋白酶A的存在可能与植物特异性插入物在活跃生长的组织中的水分吸收和溶质泄漏过程中在膜脂质转化中的拟议作用有关。这与最近提出的天冬氨酸蛋白酶前体分子的双功能作用有关，天冬氨酸蛋白酶前体分子除具有蛋白酶结构域外还具有膜不稳定结构域。在种子吸收后的最初几个小时内检测到了成熟的心蛋白酶B，但未检测到其mRNA，并在胚根出现时消失了。这种细胞外天冬氨酸蛋白酶已经被认为与细胞壁的松弛和重塑有关，它在种子萌发中的作用可能与放松胚根对甲的组织约束有关。所述的心蛋白酶A和B表达模式提示了微调的发育调节，并提示了其在胚胎后发育生理中的可能作用的分析。

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