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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Transcriptional activity and distribution of splicing machinery elements during Hyacinthus orientalis pollen tube growth
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Transcriptional activity and distribution of splicing machinery elements during Hyacinthus orientalis pollen tube growth

机译：东方风信子花粉管生长过程中的转录活性和剪接机械元件的分布

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摘要

The localization of newly formed transcripts and molecules participating in pre-mRNA splicing, i.e., small nuclear ribonucleoproteins (snRNPs) and SC35 protein, in growing pollen tubes of Hyacinthus orientalis L. were analyzed in vitro and in vivo. The results indicated that the restart of RNA synthesis occurred first in the vegetative and then in the generative nucleus of both in vitro and in vivo growing pollen tubes. Changes in RNA synthesis were accompanied by the redistribution of splicing machinery elements in both vegetative and generative nuclei of the growing pollen tube. At stages of pollen tube growth when the vegetative and generative nuclei were transcriptionally active, clear differences in the distribution pattern of the splicing system components were observed in both pollen nuclei. While both small nuclear RNA with a trimethylguanosine cap on the 5 ' end and SC35 protein were diffusely distributed in the nucleoplasm in the vegetative nucleus, the studied antigens were only present in the areas between condensed chromatin in the generative nucleus. When the transcriptional activity of both pollen nuclei could no longer be observed at later stages of pollen tube growth, snRNPs and SC35 protein were still present in the vegetative nuclei but not in the generative nuclei. We, therefore, investigated potential differences in the spatial organization of splicing system elements during pollen tube growth. They clearly reflect differences in gene expression patterns in the vegetative and the generative cells, which may be determined by the different biological roles of angiosperm male gametophyte cells.
机译：在体内和体外分析了生长于风信子的花粉管中的新形成的转录本和参与mRNA前剪接的分子（即小核糖核糖核蛋白（snRNPs）和SC35蛋白）的定位。结果表明，RNA合成的重新启动首先发生在体外和体内生长的花粉管的营养核中，然后发生在其产生核中。 RNA合成的变化伴随着剪接机械元件在生长中的花粉管的营养核和生殖核中的重新分布。在花粉管生长的阶段，当营养核和生殖核处于转录活性时，在两个花粉核中均观察到剪接系统组分分布模式的明显差异。虽然在5'端带有三甲基鸟苷帽的小核RNA和SC35蛋白均分散地分布在营养核的核质中，但研究的抗原仅存在于生殖核的染色质浓缩区之间。当在花粉管生长的后期不能再观察到两个花粉核的转录活性时，snRNPs和SC35蛋白仍存在于营养核中，而不存在于生成核中。因此，我们研究了花粉管生长过程中拼接系统元件空间组织的潜在差异。它们清楚地反映了营养细胞和生殖细胞中基因表达模式的差异，这可能由被子植物雄配子体细胞的不同生物学作用决定。

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