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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Localization of stilbene synthase in Vitis vinifera L. during berry development
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Localization of stilbene synthase in Vitis vinifera L. during berry development

机译：浆果发育过程中二苯乙烯合成酶在葡萄中的定位

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摘要

The localization of stilbene synthase (STS) (EC 2.3.1.95) in grape berry (Vitis vinifera L.) was investigated during fruit development. The berries were collected at 2, 4, 7, 11, and 15 weeks postflowering from the cultivar Nebbiolo during the 2005 and 2006 growing seasons. High-performance liquid chromatography analysis showed that berries accumulated cis- and trans-isomers of resveratrol mainly in the exocarp throughout fruit development. Immunodetection of STS protein was performed on berry extracts and sections with an antibody specifically developed against recombinant grape STS1. In agreement with resveratrol presence, STS was found in berry exocarp tissues during all stages of fruit development. The labeled epidermal cells were few and were randomly distributed, whereas nearly all the outer hypodermis cells were STS-positive. The STS signal decreased gradually from exocarp to mesocarp, where the protein was detected only occasionally. At the subcellular level, STS was found predominantly within vesicles (of varying size), along the plasma membrane and in the cell wall, suggesting protein secretion in the apoplast compartment. Despite the differences in fruit size and structure, the STS localization was the same before and after veraison, the relatively short developmental period during which the firm green berries begin to soften and change color. Nevertheless, the amount of protein detected in both exocarp and mesocarp decreased significantly in ripe berries, in agreement with the lower resveratrol content measured in the same tissues. The location of STS in exocarp cell wall is consistent with its role in synthesizing defense compounds and supports the hypothesis that a differential localization of phenylpropanoid biosynthetic machinery regulates the deposition of specific secondary products at different action sites within cells.
机译：研究了在果实发育过程中二苯乙烯合成酶（STS）（EC 2.3.1.95）在葡萄浆果（Vitis vinifera L.）中的定位。在2005年和2006年生长季节，从开花的Nebbiolo品种在开花后第2、4、7、11和15周收集浆果。高效液相色谱分析表明，浆果在整个果实发育过程中主要在外果皮中积累白藜芦醇的顺式和反式异构体。对STS蛋白的免疫检测是在浆果提取物和切片上进行的，该切片专门针对重组葡萄STS1开发。与白藜芦醇的存在一致，在果实发育的所有阶段中，浆果外果皮组织中均发现了STS。被标记的表皮细胞很少，并且是随机分布的，而几乎所有的外皮下细胞都是STS阳性的。 STS信号从外果皮到中果皮逐渐降低，在那儿仅偶尔检测到该蛋白。在亚细胞水平上，STS主要发现在囊泡（大小不等）内，沿质膜和细胞壁内，表明质外体隔室中存在蛋白质分泌。尽管果实大小和结构有所不同，但在变质前后，STS的定位是相同的，这是相对较短的发育时期，在此期间，坚硬的绿色浆果开始软化并变色。然而，成熟浆果中外果皮和中果皮中检测到的蛋白质量显着下降，这与在相同组织中测得的白藜芦醇含量较低相符。 STS在外果皮细胞壁中的位置与其在防御化合物合成中的作用一致，并支持以下假设：苯丙烷类生物合成机制的差异性定位调节了特定二级产物在细胞内不同作用位点的沉积。

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