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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)
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Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)

机译：低温下悬浮在冷冻细胞中的细胞质膜的调节（Chorispora bungeana）

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摘要

As the outermost boundary of the cell, the plasma membrane plays an important role in determining the stress resistance of organisms. To test this concept in a cryophyte, we analyzed alterations of several components in plasma membranes isolated from suspension-cultured cells of Chorispora bungeana Fisch. & C.A. Mey in response to treatment at 0 and -4 degrees C for 192 h. When compared with the controls growing at 25 degrees C, both the membrane permeability and fluidity showed recovery after the initial impairment. Linolenic acid and membrane lipid unsaturation increased by about 0.8-fold following cold treatments, although the kinetics of the increase varied with the temperatures examined. During the treatments, the plasma membrane H+-ATPase (EC 3.6.1.3) activity increased by 78.06% at 0 degrees C and 100.47% at -4 degrees C. However, the plasma membrane NADH oxidase (EC 1.6.99.3) activity only decreased when exposed to a lower temperature (-4 degrees C), and remained at 63.93% after being treated for 192 h. After the treatments, the physical properties of the plasma membranes of suspension-cultured cells, especially the -4 degrees C treated cells, were similar to those in the wild plants. These findings indicate that the specific mechanism of cold resistance of C. bungeana is tightly linked with the rapid and flexible regulation of membrane lipids and membrane-associated enzymes, which ensure the structural and functional integrity of the plasma membrane that is essential for withstanding low temperature.
机译：作为细胞的最外层边界，质膜在确定生物体的抗逆性中起重要作用。为了在冷冻剂中测试该概念，我们分析了从悬垂短孢菌悬浮培养的细胞中分离的质膜中几种成分的变化。 ＆C.A. Mey在0和-4摄氏度下治疗192小时。当与在25℃下生长的对照相比时，膜的渗透性和流动性在初始损伤后均显示出恢复。冷处理后，亚麻酸和膜脂质不饱和度增加了约0.8倍，尽管增加的动力学随所检查的温度而变化。在治疗期间，质膜H + -ATPase（EC 3.6.1.3）活性在0摄氏度下增加78.06％，在-4摄氏度下增加100.47％。但是，质膜NADH氧化酶（EC 1.6.99.3）只会降低当暴露于较低温度（-4摄氏度）时，在处理192小时后仍保持在63.93％。处理后，悬浮培养的细胞，特别是经-4℃处理的细胞的质膜的物理性质与野生植物中的相似。这些发现表明，邦氏梭菌的抗寒性的具体机制与膜脂质和膜相关酶的快速，灵活调节紧密相关，从而确保了质膜的结构和功能完整性，这对于承受低温至关重要。

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