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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Characterization, expression and subcellular distribution of a novel MFP1 (matrix attachment region-binding filament-like protein 1) in onion
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Characterization, expression and subcellular distribution of a novel MFP1 (matrix attachment region-binding filament-like protein 1) in onion

机译：洋葱中新型MFP1（基质附着区结合丝状蛋白1）的表征，表达和亚细胞分布

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摘要

MFP1 (matrix attachment region-binding filament-like protein 1) is a conserved nuclear and chloroplast DNA-binding protein encoded by a nuclear gene, well characterized in dicot species. In monocots, only a 90 kDa MFP1-related protein had been characterized in the nucleus and nuclear matrix of Allium cepa proliferating cells. We report here a novel MFP1-related nuclear protein of 80 kDa in A. cepa roots, with M-r and pI values similar to those of MFP1 proteins in dicot species, and which also displays a dual location, in the nucleus and chloroplasts of leaf cells. However, this novel protein is not a nuclear matrix component. It shows a spotted intranuclear distribution in small foci differing from the nuclear bodies containing the 90 kDa protein. In electron microscopy analysis, the intranuclear foci containing the 80 kDa MFP1 appeared as small loose structures at the periphery of condensed chromatin patches. This protein was also located in the nucleolus. It was abundant in meristematic cells, but its level fell when proliferation stopped. This different expression and distribution, and its preferential location at the boundaries between heterochromatin and euchromatin, suggest that the novel 80 kDa protein might be associated with decondensed DNA and could play a role in chromatin organization.
机译：MFP1（与基质附着区结合的丝状蛋白1）是一种保守的核和叶绿体DNA结合蛋白，由核基因编码，在双子叶植物中具有很好的特征。在单子叶植物中，葱属cepa增殖细胞的核和核基质中只有90 kDa的MFP1相关蛋白已被鉴定。我们在这里报告了一种新的与MFP1相关的核蛋白，在棕褐色根中具有80 kDa，其Mr和pI值与双子叶植物中的MFP1蛋白相似，并且在叶细胞的核和叶绿体中也显示出双重位置。但是，这种新型蛋白质不是核基质成分。它显示出小灶中有斑点的核内分布，不同于包含90 kDa蛋白的核小体。在电子显微镜分析中，含有80 kDa MFP1的核内病灶在浓缩的染色质斑块的外围表现为小的松散结构。该蛋白也位于核仁中。它富含分生组织细胞，但是当增殖停止时其水平下降。这种不同的表达和分布及其在异染色质和常染色质之间的边界处的优先位置表明，这种新型的80 kDa蛋白可能与缩合的DNA有关，并可能在染色质组织中起作用。

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