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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype
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Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype

机译：瞬时表达来自中国野葡萄葡萄的乙二醛氧化酶可以抑制易感基因型中的白粉病

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摘要

Vitis pseudoreticulata glyoxal oxidase (VpGLOX) was previously isolated from the Chinese wild vine V. pseudoreticulata accession "Baihe-35-1" during a screen for genes that are upregulated in response to infection with grapevine powdery mildew (Erysiphe necator, PM). In the present study, a possible function of VpGLOX for defense against PM was investigated using Agrobacterium-mediated transient expression. After optimizing agro-infiltration, VpGLOX was transiently overexpressed in leaves of either PM-susceptible (accession "6-12-2") or PM-resistant (accession "6-12-6") plants. The efficiency of transfection was verified using a β-glucuronidase (GUS) reporter and was found to comprise most leaf areas regardless of the initial leaf position. Upon infection with E. necator, clear differences were observed with respect to hyphal development between agro-infiltrated leaves and control groups of both, the susceptible and the resistant, genotypes. The expression of VpGLOX was followed by real-time polymerase chain reaction in both genotypes. Whereas in the susceptible host ("6-12-2") expression was found to increase only in transfected leaves and remained transient, in the resistant host ("6-12-6"), a second peak appeared later in transfected leaves, probably representing the response of the endogenous VpGLOX. The data support the interpretation that VpGLOX is sufficient to confer resistance to E. necator.
机译：先前，在筛选针对葡萄白粉病的感染而上调的基因（Erysiphe necator，PM）期间，从中国野生藤本假单胞菌登录号“ Baihe-35-1”中分离了拟白葡萄乙二醛氧化酶（VpGLOX）。在本研究中，使用农杆菌介导的瞬时表达研究了VpGLOX对抗PM的可能功能。优化农业渗透后，VpGLOX在易受PM感染（登录号“ 6-12-2”）或抗PM的植物（登录号“ 6-12-6”）的叶片中瞬时过表达。使用β-葡糖醛酸糖苷酶（GUS）报告基因验证了转染效率，发现该结构包含大部分叶面积，而与起始叶位置无关。感染了大肠杆菌后，观察到农杆菌浸润的叶片与对照组（易感基因型和抗性基因型）之间的菌丝发育有明显差异。在两种基因型中，VpGLOX的表达均通过实时聚合酶链反应进行。而在易感宿主（“ 6-12-2”）中发现表达仅在转染的叶片中增加并保持瞬时，而在抗性宿主（“ 6-12-6”）中，第二个峰值稍后出现在转染的叶片中，可能代表内源性VpGLOX的反应。数据支持以下解释：VpGLOX足以赋予对E. necator的抗性。

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