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Development and application of probes for labeling the actin cytoskeleton in living plant cells

机译:活植物细胞中肌动蛋白细胞骨架标记探针的开发与应用

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摘要

The actin cytoskeleton is one of the most important components of eukaryotic cytoskeletons. It participates in numerous crucial procedures of cells and has been studied by using various methods. The development and application of appropriate probes for actin visualization is the first and foremost step for functional analysis of actin in vivo. Since the actin cytoskeleton is a highly dynamic and sensitive structure, methods previously used to visualize actin often harm cells and cannot reveal the native state of the actin cytoskeleton in living cells. The development of labeling technologies for living plant cells, especially the emergence and application of green fluorescent protein-tagged actin markers, has provided new insights into the structure and function of the actin cytoskeleton in vivo. There has been a number of probes for actin labeling in living plant cells though they each present different advantages and defects. In this review, we discuss and compare those widely used methods for actin visualization and analysis.
机译:肌动蛋白细胞骨架是真核细胞骨架的最重要组成部分之一。它参与了许多关键的细胞程序,并已通过各种方法进行了研究。用于肌动蛋白可视化的合适探针的开发和应用是体内肌动蛋白功能分析的第一步和最重要的步骤。由于肌动蛋白细胞骨架是高度动态和敏感的结构,以前用于可视化肌动蛋白的方法通常会损害细胞,并且无法揭示活细胞中肌动蛋白细胞骨架的天然状态。活植物细胞标记技术的发展,特别是绿色荧光蛋白标记的肌动蛋白标记的出现和应用,为肌动蛋白细胞骨架在体内的结构和功能提供了新的见识。尽管在活的植物细胞中存在多种肌动蛋白标记的探针,但它们各自具有不同的优点和缺点。在这篇综述中,我们讨论并比较了那些广泛用于肌动蛋白可视化和分析的方法。

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