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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Development of the cotyledon cells during olive (Olea europaea L.) in vitro seed germination and seedling growth
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Development of the cotyledon cells during olive (Olea europaea L.) in vitro seed germination and seedling growth

机译：橄榄（Olea europaea L.）体外种子萌发和幼苗生长过程中子叶细胞的发育

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摘要

The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodies (OBs). After 3 days of in vitro germination, the presence of large PBs originated by fusion of smaller PBs was observed. It was also detected a close spatial proximity between PBs and OBs, likely as a reflection of interconnected metabolic pathways. Between the 3rd and the 12th day of germination, the formation of a large vacuolar compartment takes place accompanied by a decrease in the PBs and OBs number. This was coincident with a progressive decrease in the amount of the 11S-type seed storage proteins (SSPs), showed in situ and after Western blot analysis of crude protein extracts. After 26 days germination, the cellular organization became typical for a leaf mesophyll cell, with well-differentiated chloroplasts surrounding a large central vacuole. Our results suggest that the olive cotyledon storage reserves are mobilized gradually until the seedling becomes autotrophic. Moreover, the specific accumulation of storage proteins in the intravacuolar material suggests that these structures may operate as a shuttle for SSPs and/or products of their degradation into the cytoplasm, where finally they supply amino acids for the differentiating mesophyll cells.
机译：描述了将橄榄子叶细胞分化为叶肉细胞时发生的结构变化。使用组织学和免疫细胞学方法以及显微镜观察，我们发现在成熟胚胎的细胞中，大的电子致密蛋白体（PBs）被许多油体（OBs）包围。体外萌发3天后，观察到较大PB的存在，该较大PB源自较小PB的融合。还检测到PB和OB之间的空间接近性，可能是相互关联的代谢途径的反映。在发芽的第3天和第12天之间，形成了一个大的液泡室，并伴随着PB和OB数量的减少。原位和蛋白质印迹分析的蛋白质印迹分析表明，这与11S型种子贮藏蛋白（SSP）数量的逐渐减少相吻合。发芽26天后，细胞组织成为叶肉细胞的典型组织，叶绿体分化良好，周围有较大的中央液泡。我们的结果表明，橄榄子叶的贮藏储备是逐渐动员的，直到幼苗变得自养。此外，储液蛋白在真空中的特定积累表明，这些结构可以作为SSP和/或其降解产物进入细胞质的穿梭体，最终它们为分化的叶肉细胞提供氨基酸。

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