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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Embryogenesis in Sedum acre L.: Structural and immunocytochemical aspects of suspensor development
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Embryogenesis in Sedum acre L.: Structural and immunocytochemical aspects of suspensor development

机译：景天的胚发生：悬液发育的结构和免疫细胞化学方面

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摘要

The changes in the formation of both the actin and the microtubular cytoskeleton during the differentiation of the embryo-suspensor in Sedum acre were studied in comparison with the development of the embryo-proper. The presence and distribution of the cytoskeletal elements were examined ultrastructurally and with the light microscope using immunolabelling and rhodamine-phalloidin staining. At the globular stage of embryo development extensive array of actin filaments is present in the cytoplasm of basal cell, the microfilament bundles generally run parallel to the long axis of basal cell and pass in close to the nucleus. Microtubules form irregular bundles in the cytoplasm of the basal cell. A strongly fluorescent densely packed microtubules are present in the cytoplasmic layer adjacent to the wall separating the basal cell from the first layer of the chalazal suspensor cells. At the heart-stage of embryo development, in the basal cell, extremely dense arrays of actin materials are located near the micropylar and chalazal end of the cell. At this stage of basal cell formation, numerous actin filaments congregate around the nucleus. In the fully differentiated basal cell and micropylar haustorium, the tubulin cytoskeleton forms a dense prominent network composed of numerous cross-linked filaments. In the distal region of the basal cell, a distinct microtubular cytoskeleton with numerous microtubules is observed in the cytoplasmic layer adjacent to the wall, separating the basal cell from the first layer of the chalazal suspensor cells. The role of cytoskeleton during the development of the suspensor in S. acre is discussed.
机译：研究了与景天胚发育相比，在景天英胚分化过程中肌动蛋白和微管细胞骨架形成的变化。使用免疫标记和若丹明-鬼笔环肽染色，通过光学显微镜和超微结构检查细胞骨架元素的存在和分布。在胚胎发育的球形阶段，肌动蛋白丝的广泛排列存在于基底细胞的细胞质中，微丝束通常平行于基底细胞的长轴延伸并靠近细胞核。微管在基底细胞的细胞质中形成不规则的束。强荧光密集包装的微管存在于细胞壁中，该细胞质层与将基底细胞与第一层悬臂细胞的第一层分隔开的壁相邻。在胚胎发育的心脏阶段，在基底细胞中，极密集的肌动蛋白物质阵列位于细胞的小孔和丘脑末端附近。在基底细胞形成的这个阶段，许多肌动蛋白丝聚集在细胞核周围。在完全分化的基底细胞和小孔性脓泡中，微管蛋白细胞骨架形成由许多交联的细丝组成的密集的突出网络。在基底细胞的远端区域中，在与壁相邻的胞质层中观察到具有众多微管的独特微管细胞骨架，从而将基底细胞与第一层悬臂细胞分离。讨论了在S. acre的悬液发育过程中细胞骨架的作用。

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