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Imaging plasmodesmata

机译:影像胞浆肿

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摘要

At only 50 nm in diameter, plasmodesmata (PD) are below the limit of resolution of conventional light microscopy. Consequently, much of our current interpretation of the substructure of PD is derived from transmission electron microscopy. However, PD can be imaged with alternative techniques, including field emission scanning electron microscopy and 'super-resolution' imaging approaches such as 3D-structured illumination microscopy. This review considers the methods currently available for studying PD and focuses on the boundary between light-and electron-based imaging approaches.
机译:直径仅50 nm时,胞浆菌(PD)低于常规光学显微镜的分辨率极限。因此,我们目前对PD子结构的大部分解释都来自透射电子显微镜。但是,可以使用其他技术对PD进行成像,包括场发射扫描电子显微镜和“超分辨率”成像方法(例如3D结构的照明显微镜)。这篇综述考虑了目前可用于研究PD的方法,并着重于基于光和电子的成像方法之间的边界。

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