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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth
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Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth

机译：两种磷脂酶D抑制剂1-丁醇和N-酰基乙醇胺对体内细胞骨架组织和拟南芥幼苗生长的差异作用

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摘要

Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiology. Despite the intriguing similarities between animals and plants in NAE metabolism and perception, not much is known about the precise function of these metabolites in plant physiology. In plants, NAEs have been shown to inhibit phospholipase D alpha (PLD alpha) activity, interfere with abscisic acid-induced stomatal closure, and retard Arabidopsis seedling development. 1-Butanol, an antagonist of PLD-dependent phosphatidic acid production, was reported to induce defects in Arabidopsis seedling development that were somewhat similar to effects induced by elevated levels of NAE. This raised the possibility that the impact of NAE on seedling growth could be mediated in part via its influence on PLD activity. To begin to address this possibility, we conducted a detailed, comparative analysis of the effects of 1-butanol and N-lauroylethanolamine (NAE 12:0) on Arabidopsis root cell division, in vivo cytoskeletal organization, seed germination, and seedling growth. Although both NAE 12:0 and 1-butanol induced profound cytoskeletal and morphological alterations in seedlings, there were distinct differences in their overall effects. 1-Butanol induced more pronounced modifications in cytoskeletal organization, seedling growth, and cell division at concentrations severalfold higher than NAE 12:0. We propose that these compounds mediate their differential effects on cellular organization and seedling growth, in part through the differential modulation of specific PLD isoforms.
机译：植物的发育受多种来自多种代谢途径的化学物质的调控。但是，我们对细胞中许多这些代谢产物的生物学作用和功能知之甚少。 N-酰基乙醇胺（NAE）是一组脂质介体，在哺乳动物生理中起重要作用。尽管动植物之间在NAE代谢和知觉方面有着令人着迷的相似之处，但对于这些代谢物在植物生理学中的精确功能知之甚少。在植物中，NAE已显示出抑制磷脂酶Dα（PLDα）的活性，干扰脱落酸诱导的气孔闭合并阻碍拟南芥幼苗的发育。据报道，1-丁醇是PLD依赖的磷脂酸产生的拮抗剂，可诱​​导拟南芥幼苗发育中的缺陷，其与NAE水平升高所引起的效应有些相似。这增加了NAE对幼苗生长的影响可能部分地通过其对PLD活性的影响来介导的可能性。为了解决这种可能性，我们对1-丁醇和N-月桂酰乙醇胺（NAE 12：0）对拟南芥根细胞分裂，体内细胞骨架组织，种子萌发和幼苗生长的影响进行了详细的比较分析。尽管NAE 12：0和1-丁醇均可诱导幼苗的细胞骨架和形态发生深远的变化，但它们的整体效果却存在明显差异。 1-丁醇在比NAE 12：0高几倍的浓度下诱导了细胞骨架组织，幼苗生长和细胞分裂的更明显修饰。我们建议这些化合物介导其对细胞组织和幼苗生长的差异作用，部分是通过特定PLD同工型的差异调节来实现的。

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