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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Acrylamide inhibits gravitropism and affects microtubules in rice coleoptiles
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Acrylamide inhibits gravitropism and affects microtubules in rice coleoptiles

机译:丙烯酰胺可抑制重力并影响胚芽鞘中的微管

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摘要

To find components which participate in gravitropic signal transmission, we screened different cell biological inhibitors for their effect on gravitropic bending of rice coleoptiles. Acrylamide, which is known to affect intermediate filaments in mammalian cells, strongly inhibited gravitropic bending at concentrations that did not inhibit growth of coleoptile segments. This inhibition was reversible. Investigating the acrylamide effect further, we found that it interferes with an event that occurs around 15 min after the onset of stimulation. We also observed that acrylamide inhibits polar indolyl-3-acetic acid transport. Furthermore, acrylamide efficiently eliminated microtubules, whereas actin filaments remained intact. To our knowledge this is the first report of effects of monoacrylamide in plant cells.
机译:为了找到参与重力信号传递的成分,我们筛选了不同的细胞生物抑制剂对它们对水稻胚芽鞘的重力弯曲的影响。已知会影响哺乳动物细胞中间丝的丙烯酰胺在不抑制胚芽鞘节段生长的浓度下强烈抑制重力弯曲。这种抑制作用是可逆的。进一步研究丙烯酰胺的作用,我们发现它会干扰刺激发生后15分钟左右发生的事件。我们还观察到丙烯酰胺抑制了吲哚基-3-乙酸的极性转运。此外,丙烯酰胺有效地消除了微管,而肌动蛋白丝保持完整。据我们所知,这是单丙烯酰胺在植物细胞中作用的首次报道。

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