...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Involvement of gibberellin in tracheary element differentiation and lignification in Zinnia elegans xylogenic culture
【24h】

Involvement of gibberellin in tracheary element differentiation and lignification in Zinnia elegans xylogenic culture

机译：赤霉素在百日草成木培养物中气管元素分化和木质化中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gibberellin (GA) is considered an important growth regulator involved in many aspects of plant development. However, little is known about the relationship between GA and lignification. In this study, we analyzed the role of GA in tracheary element (TE) differentiation and lignification using a Zinnia elegans xylogenic culture. When gibberellic acid-3 (GA(3)) was exogenously supplied, a slight increase in the frequency of TE differentiation and a remarkable increase in lignin content were observed. Computer image analysis of individual TEs showed that the lignification level of each TE was significantly increased in the culture treated with GA(3) compared with those of the control. In contrast, suppression of TE differentiation and lignification was observed when GA biosynthesis was inhibited by ancymidol, paclobutrazol, or uniconazole. This suppression was restored by the addition of GA(3). These results suggest that GA plays an important role in TE differentiation, and even more so in lignification. When conditioned medium obtained after 120 h of control culture was analyzed by high-performance liquid chromatography, many lignin precursors were detected. However, these lignin precursors were greatly reduced in the GA-treated culture. This result suggests that GA promotes lignification by activating the polymerization of lignin precursors. Gibberellin (GA) is considered an important growth regulator involved in many aspects of plant development. However, little is known about the relationship between GA and lignification. In this study, we analyzed the role of GA in tracheary element (TE) differentiation and lignification using a Zinnia elegans xylogenic culture. When gibberellic acid-3 (GA(3)) was exogenously supplied, a slight increase in the frequency of TE differentiation and a remarkable increase in lignin content were observed. Computer image analysis of individual TEs showed that the lignification level of each TE was significantly increased in the culture treated with GA(3) compared with those of the control. In contrast, suppression of TE differentiation and lignification was observed when GA biosynthesis was inhibited by ancymidol, paclobutrazol, or uniconazole. This suppression was restored by the addition of GA(3). These results suggest that GA plays an important role in TE differentiation, and even more so in lignification. When conditioned medium obtained after 120 h of control culture was analyzed by high-performance liquid chromatography, many lignin precursors were detected. However, these lignin precursors were greatly reduced in the GA-treated culture. This result suggests that GA promotes lignification by activating the polymerization of lignin precursors.
机译：赤霉素（GA）被认为是重要的生长调节剂，参与植物发育的许多方面。然而，关于GA和木质化之间的关系知之甚少。在这项研究中，我们分析了使用百日草线虫的木本生文化中GA在气管元件（TE）分化和木质化中的作用。当外源供应赤霉素3（GA（3））时，观察到TE分化的频率略有增加，木质素含量显着增加。单个TE的计算机图像分析表明，与对照相比，GA（3）处理的培养物中每个TE的木质化水平显着提高。相反，当抗生物素被噻虫醇，多效唑或烯效唑抑制时，可抑制TE的分化和木质化。通过添加GA（3）恢复了这种抑制。这些结果表明，GA在TE分化中起着重要作用，甚至在木质化中也起着重要作用。通过高效液相色谱分析对照培养120小时后获得的条件培养基时，检测到许多木质素前体。然而，这些木质素前体在GA处理的培养物中被大大减少。该结果表明GA通过激活木质素前体的聚合来促进木质化。赤霉素（GA）被认为是重要的生长调节剂，参与植物发育的许多方面。但是，关于GA和木质化之间的关系知之甚少。在这项研究中，我们分析了GA在百日草线虫异木培养中在气管元件（TE）分化和木质化中的作用。当外源供应赤霉素3（GA（3））时，观察到TE分化的频率略有增加，木质素含量显着增加。单个TE的计算机图像分析表明，与对照相比，GA（3）处理的培养物中每个TE的木质化水平显着提高。相反，当抗生物素被噻虫醇，多效唑或烯效唑抑制时，可抑制TE的分化和木质化。通过添加GA（3）恢复了这种抑制。这些结果表明，GA在TE分化中起着重要作用，甚至在木质化中也起着重要作用。通过高效液相色谱分析对照培养120小时后获得的条件培养基时，检测到许多木质素前体。然而，这些木质素前体在GA处理的培养物中被大大减少。该结果表明GA通过活化木质素前体的聚合来促进木质化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号